Calcinueurin is a calcium ion-dependent, calmodulin-stimulated protein phosphatase

Nicholas K. Tonks, Philip Cohen

    Research output: Contribution to journal › Article

    34 Citations (Scopus)

    Abstract

    A Ca2+-dependent, calmodulin-stimulated protein phosphatase (EC 3.1.3.16) is known to be associated with calcineurin, a major calmodulin binding protein in brain. The protein phosphatase activity has now been shown to be retained by a substrate affinity column (thiophosphorylated myosin P-light chain Sepharose) in the presence of Ca2+, and to be eluted specifically with EGTA. Calcineurin behaved identically. This establishes that calcineurin is the Ca2+-dependent protein phosphatase, and that interaction of Ca2+ with the B-subunit is essential for substrate binding.

    Original languageEnglish
    Pages (from-to)191-193
    Number of pages3
    JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
    Volume747
    Issue number1-2
    DOIs
    Publication statusPublished - 14 Sep 1983

    Fingerprint

    Calcineurin
    Phosphoprotein Phosphatases
    Calmodulin
    Ions
    Calcium
    Calmodulin-Binding Proteins
    Myosin Light Chains
    Egtazic Acid
    Substrates
    Myosins
    Sepharose
    Brain

    Keywords

    • Affinity chromatography
    • Ca-dependent protein phosphatase
    • Calcineurin
    • Calmodulin binding protein

    Cite this

    Tonks, Nicholas K. ; Cohen, Philip. / Calcinueurin is a calcium ion-dependent, calmodulin-stimulated protein phosphatase. In: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular. 1983 ; Vol. 747, No. 1-2. pp. 191-193.
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    Tonks, NK & Cohen, P 1983, 'Calcinueurin is a calcium ion-dependent, calmodulin-stimulated protein phosphatase', Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, vol. 747, no. 1-2, pp. 191-193. https://doi.org/10.1016/0167-4838(83)90140-1

    Calcinueurin is a calcium ion-dependent, calmodulin-stimulated protein phosphatase. / Tonks, Nicholas K.; Cohen, Philip.

    In: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, Vol. 747, No. 1-2, 14.09.1983, p. 191-193.

    Research output: Contribution to journal › Article

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    AB - A Ca2+-dependent, calmodulin-stimulated protein phosphatase (EC 3.1.3.16) is known to be associated with calcineurin, a major calmodulin binding protein in brain. The protein phosphatase activity has now been shown to be retained by a substrate affinity column (thiophosphorylated myosin P-light chain Sepharose) in the presence of Ca2+, and to be eluted specifically with EGTA. Calcineurin behaved identically. This establishes that calcineurin is the Ca2+-dependent protein phosphatase, and that interaction of Ca2+ with the B-subunit is essential for substrate binding.

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    Tonks NK, Cohen P. Calcinueurin is a calcium ion-dependent, calmodulin-stimulated protein phosphatase. Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular. 1983 Sep 14;747(1-2):191-193. https://doi.org/10.1016/0167-4838(83)90140-1

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