TY - JOUR
T1 - Calcinueurin is a calcium ion-dependent, calmodulin-stimulated protein phosphatase
AU - Tonks, Nicholas K.
AU - Cohen, Philip
PY - 1983/9/14
Y1 - 1983/9/14
N2 - A Ca2+-dependent, calmodulin-stimulated protein phosphatase (EC 3.1.3.16) is known to be associated with calcineurin, a major calmodulin binding protein in brain. The protein phosphatase activity has now been shown to be retained by a substrate affinity column (thiophosphorylated myosin P-light chain Sepharose) in the presence of Ca2+, and to be eluted specifically with EGTA. Calcineurin behaved identically. This establishes that calcineurin is the Ca2+-dependent protein phosphatase, and that interaction of Ca2+ with the B-subunit is essential for substrate binding.
AB - A Ca2+-dependent, calmodulin-stimulated protein phosphatase (EC 3.1.3.16) is known to be associated with calcineurin, a major calmodulin binding protein in brain. The protein phosphatase activity has now been shown to be retained by a substrate affinity column (thiophosphorylated myosin P-light chain Sepharose) in the presence of Ca2+, and to be eluted specifically with EGTA. Calcineurin behaved identically. This establishes that calcineurin is the Ca2+-dependent protein phosphatase, and that interaction of Ca2+ with the B-subunit is essential for substrate binding.
KW - Affinity chromatography
KW - Ca-dependent protein phosphatase
KW - Calcineurin
KW - Calmodulin binding protein
UR - http://www.scopus.com/inward/record.url?scp=0021030632&partnerID=8YFLogxK
U2 - 10.1016/0167-4838(83)90140-1
DO - 10.1016/0167-4838(83)90140-1
M3 - Article
C2 - 6309241
AN - SCOPUS:0021030632
VL - 747
SP - 191
EP - 193
JO - Biochimica et Biophysica Acta. Protein Structure and Molecular Enzymology
JF - Biochimica et Biophysica Acta. Protein Structure and Molecular Enzymology
SN - 0167-4838
IS - 1-2
ER -