Calcinueurin is a calcium ion-dependent, calmodulin-stimulated protein phosphatase

Nicholas K. Tonks; Philip Cohen

doi:10.1016/0167-4838(83)90140-1

Calcinueurin is a calcium ion-dependent, calmodulin-stimulated protein phosphatase

Nicholas K. Tonks, Philip Cohen

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

A Ca²⁺-dependent, calmodulin-stimulated protein phosphatase (EC 3.1.3.16) is known to be associated with calcineurin, a major calmodulin binding protein in brain. The protein phosphatase activity has now been shown to be retained by a substrate affinity column (thiophosphorylated myosin P-light chain Sepharose) in the presence of Ca²⁺, and to be eluted specifically with EGTA. Calcineurin behaved identically. This establishes that calcineurin is the Ca²⁺-dependent protein phosphatase, and that interaction of Ca²⁺ with the B-subunit is essential for substrate binding.

Original language	English
Pages (from-to)	191-193
Number of pages	3
Journal	Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume	747
Issue number	1-2
DOIs	https://doi.org/10.1016/0167-4838(83)90140-1
Publication status	Published - 14 Sept 1983

Keywords

Affinity chromatography
Ca-dependent protein phosphatase
Calcineurin
Calmodulin binding protein

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology
Structural Biology

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abstract = "A Ca2+-dependent, calmodulin-stimulated protein phosphatase (EC 3.1.3.16) is known to be associated with calcineurin, a major calmodulin binding protein in brain. The protein phosphatase activity has now been shown to be retained by a substrate affinity column (thiophosphorylated myosin P-light chain Sepharose) in the presence of Ca2+, and to be eluted specifically with EGTA. Calcineurin behaved identically. This establishes that calcineurin is the Ca2+-dependent protein phosphatase, and that interaction of Ca2+ with the B-subunit is essential for substrate binding.",

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AU - Cohen, Philip

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AB - A Ca2+-dependent, calmodulin-stimulated protein phosphatase (EC 3.1.3.16) is known to be associated with calcineurin, a major calmodulin binding protein in brain. The protein phosphatase activity has now been shown to be retained by a substrate affinity column (thiophosphorylated myosin P-light chain Sepharose) in the presence of Ca2+, and to be eluted specifically with EGTA. Calcineurin behaved identically. This establishes that calcineurin is the Ca2+-dependent protein phosphatase, and that interaction of Ca2+ with the B-subunit is essential for substrate binding.

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KW - Ca-dependent protein phosphatase

KW - Calcineurin

KW - Calmodulin binding protein

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Calcinueurin is a calcium ion-dependent, calmodulin-stimulated protein phosphatase

Abstract

Keywords

ASJC Scopus subject areas

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