Calcium dependence of A-CAM function in human renal epithelium

L. R. Biddlestone, S. Fleming

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)

    Abstract

    Previously we have used immunocytochemistry to identify A-CAM as a major cell adhesion molecule in human renal tubular epithelium. In this study, we demonstrate the calcium dependence of cell-cell adhesion and A-CAM function in cultured human renal epithelial cells. Separation of adjacent cells was seen within 1 min following sequestration of calcium from the culture medium with EDTA. This loss of intracellular adhesion in low calcium conditions was accompanied by the disappearance of A-CAM immunoreactivity. The changes were reversible and on return to normal calcium concentrations, A-CAM immunoreactivity reappeared and cell adhesion was re-established. The time course of this recovery was slow, taking 2-3 h. These results demonstrate the calcium dependence of cell adhesion in cultured human renal epithelial cells and confirm the adhesive function of the immunoreactive A-CAM molecule.
    Original languageEnglish
    Pages (from-to)163-9
    Number of pages7
    JournalJournal of Pathology
    Volume166
    Issue number2
    DOIs
    Publication statusPublished - 1992

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