Calmodulin-dependent protein kinase kinase-β is an alternative upstream kinase for AMP-activated protein kinase

Simon A. Hawley, David A. Pan, Kirsty J. Mustard, Louise Ross, Jenny Bain, Arthur M. Edelman, Bruno G. Frenguelli, D. Grahame Hardie

    Research output: Contribution to journalArticle

    1110 Citations (Scopus)

    Abstract

    The AMP-activated protein kinase (AMPK) is a critical regulator of energy balance at both the cellular and whole-body levels. Two upstream kinases have been reported to activate AMPK in cell-free assays, i.e., the tumor suppressor LKB1 and calmodulin-dependent protein kinase kinase. However, evidence that this is physiologically relevant currently only exists for LKB1. We now report that there is a significant basal activity and phosphorylation of AMPK in LKB1-deficient cells that can be stimulated by Ca2+ ionophores, and studies using the CaMKK inhibitor STO-609 and isoform-specific siRNAs show that CaMKKß is required for this effect. CaMKKß also activates AMPK much more rapidly than CaMKKa in cell-free assays. K+-induced depolarization in rat cerebrocortical slices, which increases intracellular Ca2+ without disturbing cellular adenine nucleotide levels, activates AMPK, and this is blocked by STO-609. Our results suggest a potential Ca2+-dependent neuroprotective pathway involving phosphorylation and activation of AMPK by CaMKKß.
    Original languageEnglish
    Pages (from-to)9-19
    Number of pages11
    JournalCell Metabolism
    Volume2
    Issue number1
    DOIs
    Publication statusPublished - Jul 2005

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