cAMP production by adenylyl cyclase G induces prespore differentiation in Dictyostelium slugs

Elisa Alvarez-Curto, Shweta Saran, Marcel Meima, Jenny Zobel, Claire Scott, Pauline Schaap

    Research output: Contribution to journalArticle

    26 Citations (Scopus)

    Abstract

    Encystation and sporulation are crucial developmental transitions for solitary and social amoebae, respectively. Whereas little is known of encystation, sporulation requires both extra- and intracellular cAMP. After aggregation of social amoebae, extracellular cAMP binding to surface receptors and intracellular cAMP binding to cAMP-dependent protein kinase (PKA) act together to induce prespore differentiation. Later, a second episode of PKA activation triggers spore maturation. Adenylyl cyclase B (ACB) produces cAMP for maturation, but the cAMP source for prespore induction is unknown. We show that adenylyl cyclase G (ACG) protein is upregulated in prespore tissue after aggregation. acg null mutants show reduced prespore differentiation, which becomes very severe when ACB is also deleted. ACB is normally expressed in prestalk cells, but is upregulated in the prespore region of acg null structures. These data show that ACG induces prespore differentiation in wild-type cells, with ACB capable of partially taking over this function in its absence.
    Original languageEnglish
    Pages (from-to)959-966
    Number of pages8
    JournalDevelopment
    Volume134
    Issue number5
    DOIs
    Publication statusPublished - 2007

    Keywords

    • Pattern formation
    • Cell-type specification
    • Sporulation
    • Encystation
    • Adenylyl cyclase G
    • Dictostelium discoideum

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