Campylobacter jejuni lipooligosaccharides modulate dendritic cell-mediated T cell polarization in a sialic acid linkage-dependent manner

Marieke Bax, Mark L. Kuijf, Astrid P. Heikema, Wouter van Rijs, Sven C. M. Bruijns, Juan J. Garcia-Vallejo, Paul R. Crocker, Bart C. Jacobs, Sandra J. van Vliet, Yvette van Kooyk

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    66 Citations (Scopus)


    Carbohydrate mimicry between Campylobacter jejuni lipooligosaccharides (LOS) and host neural ganglio-sides plays a crucial role in the pathogenesis of Guillain-Barre syndrome (GBS). Campylobacter jejuni LOS may mimic various gangliosides, which affects the immunogenicity and the type of neurological deficits in GBS patients. Previous studies have shown the interaction of LOS with sialic acid-specific siglec receptors, although the functional consequences remain unknown. Cells that express high levels of siglecs include dendritic cells (DCs), which are crucial for initiation and differentiation of immune responses. We confirm that alpha 2,3-sialylated GD1a/GM1a mimic and alpha 2,8-sialylated GD1c mimic LOS structures interact with recombinant Sn and siglec-7, respectively. Although the linkage of the terminal sialic acid of LOS did not regulate expression of DC maturation markers, it displayed clear opposite expression levels of interleukin-12 (IL-12) and OX40L, molecules involved in DC-mediated Th cell differentiation. Accordingly, targeting DC-expressed siglec-7 with alpha 2,8-linked sialylated LOS resulted in Th1 responses, whereas Th2 responses were induced by targeting with LOS containing alpha 2,3-linked sialic acid. Thus, our data demonstrate for the first time that depending on the sialylated composition of Campylobacter jejuni LOS, specific Th differentiation programs are initiated, possibly through targeting of distinct DC-expressed siglecs.

    Original languageEnglish
    Pages (from-to)2681-2689
    Number of pages9
    JournalInfection and Immunity
    Issue number7
    Publication statusPublished - Jul 2011


    • Guillain-Barre syndrome
    • Immunoglobulin-like lectins
    • CD33-related siglecs
    • Human monocytes
    • Immune system
    • In vitro
    • Binding
    • Expression
    • Receptor
    • Lipopolysaccharide


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