Carbon nanotube-enhanced cell electropermeabilisation

Vittoria Raffa, Gianni Ciofani, Orazio Vittorio, Virginia Pensabene, Alfred Cuschieri

    Research output: Contribution to journalArticle

    22 Citations (Scopus)

    Abstract

    The use of controlled electric fields to facilitate cell permeabilisation for enhanced cellular uptake of molecules is well established. The main limitation to the application of this technology in clinical practice is the requirements of high voltages which cause significant cell death in the target tissue. This paper presents a new modality for cell electro-permeabilisation based on the use of carbon nanotubes (CNTs) and external static electric fields. An explanation of the results based on the dielectric response of multiwall CNTs (MWCNTs) to these electric fields is proposed. The experimental data obtained indicate that this method of CNT-enhanced electro-permeabilisation provides an effective means of lowering the electric field voltage required for repairable cell electro-permeabilisation to below 50 V/cm and with an efficiency exceeding 80%. (C) 2009 Elsevier B.V. All rights reserved.

    Original languageEnglish
    Pages (from-to)136-141
    Number of pages6
    JournalBioelectrochemistry
    Volume79
    Issue number1
    DOIs
    Publication statusPublished - Aug 2010

    Keywords

    • Carbon nanotubes
    • Static electric fields
    • Cell electropermeabilization
    • IN-VIVO
    • ELECTRIC-FIELDS
    • ELECTROPORATION
    • DNA
    • PULSES
    • MOUSE
    • ELECTROCHEMOTHERAPY
    • SUSPENSION
    • OXIDATION
    • DELIVERY

    Cite this

    Raffa, Vittoria ; Ciofani, Gianni ; Vittorio, Orazio ; Pensabene, Virginia ; Cuschieri, Alfred. / Carbon nanotube-enhanced cell electropermeabilisation. In: Bioelectrochemistry. 2010 ; Vol. 79, No. 1. pp. 136-141.
    @article{6e4df34c96ab479d8a446e19feded0a6,
    title = "Carbon nanotube-enhanced cell electropermeabilisation",
    abstract = "The use of controlled electric fields to facilitate cell permeabilisation for enhanced cellular uptake of molecules is well established. The main limitation to the application of this technology in clinical practice is the requirements of high voltages which cause significant cell death in the target tissue. This paper presents a new modality for cell electro-permeabilisation based on the use of carbon nanotubes (CNTs) and external static electric fields. An explanation of the results based on the dielectric response of multiwall CNTs (MWCNTs) to these electric fields is proposed. The experimental data obtained indicate that this method of CNT-enhanced electro-permeabilisation provides an effective means of lowering the electric field voltage required for repairable cell electro-permeabilisation to below 50 V/cm and with an efficiency exceeding 80{\%}. (C) 2009 Elsevier B.V. All rights reserved.",
    keywords = "Carbon nanotubes, Static electric fields, Cell electropermeabilization, IN-VIVO, ELECTRIC-FIELDS, ELECTROPORATION, DNA, PULSES, MOUSE, ELECTROCHEMOTHERAPY, SUSPENSION, OXIDATION, DELIVERY",
    author = "Vittoria Raffa and Gianni Ciofani and Orazio Vittorio and Virginia Pensabene and Alfred Cuschieri",
    year = "2010",
    month = "8",
    doi = "10.1016/j.bioelechem.2009.10.006",
    language = "English",
    volume = "79",
    pages = "136--141",
    journal = "Bioelectrochemistry",
    issn = "1567-5394",
    publisher = "Elsevier",
    number = "1",

    }

    Carbon nanotube-enhanced cell electropermeabilisation. / Raffa, Vittoria; Ciofani, Gianni; Vittorio, Orazio; Pensabene, Virginia; Cuschieri, Alfred.

    In: Bioelectrochemistry, Vol. 79, No. 1, 08.2010, p. 136-141.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Carbon nanotube-enhanced cell electropermeabilisation

    AU - Raffa, Vittoria

    AU - Ciofani, Gianni

    AU - Vittorio, Orazio

    AU - Pensabene, Virginia

    AU - Cuschieri, Alfred

    PY - 2010/8

    Y1 - 2010/8

    N2 - The use of controlled electric fields to facilitate cell permeabilisation for enhanced cellular uptake of molecules is well established. The main limitation to the application of this technology in clinical practice is the requirements of high voltages which cause significant cell death in the target tissue. This paper presents a new modality for cell electro-permeabilisation based on the use of carbon nanotubes (CNTs) and external static electric fields. An explanation of the results based on the dielectric response of multiwall CNTs (MWCNTs) to these electric fields is proposed. The experimental data obtained indicate that this method of CNT-enhanced electro-permeabilisation provides an effective means of lowering the electric field voltage required for repairable cell electro-permeabilisation to below 50 V/cm and with an efficiency exceeding 80%. (C) 2009 Elsevier B.V. All rights reserved.

    AB - The use of controlled electric fields to facilitate cell permeabilisation for enhanced cellular uptake of molecules is well established. The main limitation to the application of this technology in clinical practice is the requirements of high voltages which cause significant cell death in the target tissue. This paper presents a new modality for cell electro-permeabilisation based on the use of carbon nanotubes (CNTs) and external static electric fields. An explanation of the results based on the dielectric response of multiwall CNTs (MWCNTs) to these electric fields is proposed. The experimental data obtained indicate that this method of CNT-enhanced electro-permeabilisation provides an effective means of lowering the electric field voltage required for repairable cell electro-permeabilisation to below 50 V/cm and with an efficiency exceeding 80%. (C) 2009 Elsevier B.V. All rights reserved.

    KW - Carbon nanotubes

    KW - Static electric fields

    KW - Cell electropermeabilization

    KW - IN-VIVO

    KW - ELECTRIC-FIELDS

    KW - ELECTROPORATION

    KW - DNA

    KW - PULSES

    KW - MOUSE

    KW - ELECTROCHEMOTHERAPY

    KW - SUSPENSION

    KW - OXIDATION

    KW - DELIVERY

    U2 - 10.1016/j.bioelechem.2009.10.006

    DO - 10.1016/j.bioelechem.2009.10.006

    M3 - Article

    VL - 79

    SP - 136

    EP - 141

    JO - Bioelectrochemistry

    JF - Bioelectrochemistry

    SN - 1567-5394

    IS - 1

    ER -