TY - JOUR
T1 - Casein kinase 1 isoform 2 is essential for bloodstream form Trypanosoma brucei
AU - Urbaniak, Michael D.
N1 - MEDLINE® is the source for the MeSH terms of this document.
PY - 2009/8
Y1 - 2009/8
N2 - Induction of RNA interference targeted against casein kinase 1 isoform 2 (TbCK1.2, Tb927.5.800) in bloodstream form Trypanosoma brucei in vitro results in rapid cessation of growth, gross morphological changes, multinucleation and ultimately cell death. A null mutant of the highly homologous casein kinase 1 isoform 1 (Tb927.5.790) in bloodstream form T. brucei displays no growth or morphological phenotype in vitro. A truncated form of TbCK1.2 expressed in Escherichia coli as a GST fusion produces catalytically active recombinant protein, facilitating screening for small molecule inhibitors. These data show that TbCK1.2 is an attractive target for anti-trypanosomal drug discovery.
AB - Induction of RNA interference targeted against casein kinase 1 isoform 2 (TbCK1.2, Tb927.5.800) in bloodstream form Trypanosoma brucei in vitro results in rapid cessation of growth, gross morphological changes, multinucleation and ultimately cell death. A null mutant of the highly homologous casein kinase 1 isoform 1 (Tb927.5.790) in bloodstream form T. brucei displays no growth or morphological phenotype in vitro. A truncated form of TbCK1.2 expressed in Escherichia coli as a GST fusion produces catalytically active recombinant protein, facilitating screening for small molecule inhibitors. These data show that TbCK1.2 is an attractive target for anti-trypanosomal drug discovery.
UR - http://www.scopus.com/inward/record.url?scp=67349226143&partnerID=8YFLogxK
U2 - 10.1016/j.molbiopara.2009.03.001
DO - 10.1016/j.molbiopara.2009.03.001
M3 - Article
AN - SCOPUS:67349226143
SN - 0166-6851
VL - 166
SP - 183
EP - 185
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
IS - 2
ER -