Casein kinase 1 isoform 2 is essential for bloodstream form Trypanosoma brucei

Michael D. Urbaniak

    Research output: Contribution to journalArticle

    22 Citations (Scopus)
    1 Downloads (Pure)

    Abstract

    Induction of RNA interference targeted against casein kinase 1 isoform 2 (TbCK1.2, Tb927.5.800) in bloodstream form Trypanosoma brucei in vitro results in rapid cessation of growth, gross morphological changes, multinucleation and ultimately cell death. A null mutant of the highly homologous casein kinase 1 isoform 1 (Tb927.5.790) in bloodstream form T. brucei displays no growth or morphological phenotype in vitro. A truncated form of TbCK1.2 expressed in Escherichia coli as a GST fusion produces catalytically active recombinant protein, facilitating screening for small molecule inhibitors. These data show that TbCK1.2 is an attractive target for anti-trypanosomal drug discovery.
    Original languageEnglish
    Pages (from-to)183-185
    Number of pages3
    JournalMolecular and Biochemical Parasitology
    Volume166
    Issue number2
    DOIs
    Publication statusPublished - Aug 2009

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