Casein kinase 1 isoform 2 is essential for bloodstream form Trypanosoma brucei

TY - JOUR

T1 - Casein kinase 1 isoform 2 is essential for bloodstream form Trypanosoma brucei

AU - Urbaniak, Michael D.

N1 - MEDLINE® is the source for the MeSH terms of this document.

PY - 2009/8

Y1 - 2009/8

N2 - Induction of RNA interference targeted against casein kinase 1 isoform 2 (TbCK1.2, Tb927.5.800) in bloodstream form Trypanosoma brucei in vitro results in rapid cessation of growth, gross morphological changes, multinucleation and ultimately cell death. A null mutant of the highly homologous casein kinase 1 isoform 1 (Tb927.5.790) in bloodstream form T. brucei displays no growth or morphological phenotype in vitro. A truncated form of TbCK1.2 expressed in Escherichia coli as a GST fusion produces catalytically active recombinant protein, facilitating screening for small molecule inhibitors. These data show that TbCK1.2 is an attractive target for anti-trypanosomal drug discovery.

AB - Induction of RNA interference targeted against casein kinase 1 isoform 2 (TbCK1.2, Tb927.5.800) in bloodstream form Trypanosoma brucei in vitro results in rapid cessation of growth, gross morphological changes, multinucleation and ultimately cell death. A null mutant of the highly homologous casein kinase 1 isoform 1 (Tb927.5.790) in bloodstream form T. brucei displays no growth or morphological phenotype in vitro. A truncated form of TbCK1.2 expressed in Escherichia coli as a GST fusion produces catalytically active recombinant protein, facilitating screening for small molecule inhibitors. These data show that TbCK1.2 is an attractive target for anti-trypanosomal drug discovery.

UR - https://www.scopus.com/pages/publications/67349226143

U2 - 10.1016/j.molbiopara.2009.03.001

DO - 10.1016/j.molbiopara.2009.03.001

M3 - Article

AN - SCOPUS:67349226143

SN - 0166-6851

VL - 166

SP - 183

EP - 185

JO - Molecular and Biochemical Parasitology

JF - Molecular and Biochemical Parasitology

IS - 2

ER -