cDNA cloning and expression of a potato (Solanum tuberosum) invertase

Peter E. Hedley, Gordon C. Machray (Lead / Corresponding author), Howard V. Davies, Lindsay Burch, Robbie Waugh

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A cDNA clone encoding an invertase isoenzyme has been isolated from a potato leaf cDNA library. The deduced amino acid sequence shows significant similarities to previously characterised invertases. The highest degree of overall similarity, including the signal peptide sequence, is to carrot cell wall invertase, suggesting that the potato gene encodes an apoplastic enzyme. Expression of the gene, as determined by RT-PCR, is detected in stem and leaf tissue, and at lower levels in tuber, but is absent from roots.

Original languageEnglish
Pages (from-to)917-922
Number of pages6
JournalPlant Molecular Biology
Volume22
Issue number5
DOIs
Publication statusPublished - 1 Aug 1993

Keywords

  • β-fructofuranosidase
  • β-fructosidase
  • RT-PCR

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