Cell cycle-independent furrowing triggered by phosphomimetic mutations of the INCENP STD motif requires Plk1

Diana Papini, Xavier Fant, Hiromi Ogawa, Nathalie Desban, Kumiko Samejima, Omid Feizbakhsh, Bilge Askin, Tony Ly, William C. Earnshaw (Lead / Corresponding author), Sandrine Ruchaud (Lead / Corresponding author)

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    5 Citations (Scopus)
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    Abstract

    Timely and precise control of Aurora B kinase, the chromosomal passenger complex (CPC) catalytic subunit, is essential for accurate chromosome segregation and cytokinesis. Post-translational modifications of CPC subunits are directly involved in controlling Aurora B activity. Here, we identified a highly conserved acidic STD-rich motif of INCENP that is phosphorylated during mitosis in vivo and by Plk1 in vitro and is involved in controlling Aurora B activity. By using an INCENP conditional-knockout cell line, we show that impairing the phosphorylation status of this region disrupts chromosome congression and induces cytokinesis failure. In contrast, mimicking constitutive phosphorylation not only rescues cytokinesis but also induces ectopic furrows and contractile ring formation in a Plk1- and ROCK1-dependent manner independent of cell cycle and microtubule status. Our experiments identify the phospho-regulation of the INCENP STD motif as a novel mechanism that is key for chromosome alignment and cytokinesis.
    Original languageEnglish
    Article numberjcs234401
    Number of pages16
    JournalJournal of Cell Science
    Volume132
    Issue number21
    DOIs
    Publication statusPublished - 6 Nov 2019

    Keywords

    • CPC
    • INCENP
    • Furrow initiation
    • Aurora B
    • Plk1
    • Mitosis

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