Projects per year
Abstract
AMP-activated protein kinase (AMPK) is an energy sensor that is activated by increases in the cellular AMP/ATP and ADP/ATP ratios by three mechanisms: (1) allosteric activation, (2) promotion of phosphorylation at Thr172 on the α subunit by upstream kinases, and (3) inhibition of dephosphorylation of Thr172 by protein phosphatases. All of these effects are triggered by the binding of AMP or ADP at one or more of three sites on the γ subunit, where they displace ATP. AMPK is also activated by ligands that bind in the ADaM site, which is located between the α and β subunits. In this chapter we describe cell-free assays that can be used to study these varied activation mechanisms.
Original language | English |
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Title of host publication | AMPK |
Subtitle of host publication | Methods and Protocols |
Editors | Dietbert Neumann, Benoit Viollet |
Place of Publication | New York |
Publisher | Humana Press |
Pages | 69-86 |
Number of pages | 18 |
ISBN (Electronic) | 9781493975983 |
ISBN (Print) | 9781493975976 |
DOIs | |
Publication status | Published - 2018 |
Publication series
Name | Methods in Molecular Biology |
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Publisher | Humana Press |
Volume | 1732 |
ISSN (Print) | 1064-3745 |
ISSN (Electronic) | 1940-6029 |
Keywords
- Allosteric activation
- AMP-activated protein kinase
- AMPK
- Dephosphorylation
- Kinase assay
- Phosphorylation
ASJC Scopus subject areas
- Molecular Biology
- Genetics
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Dive into the research topics of 'Cell-Free Assays to Measure Effects of Regulatory Ligands on AMPK'. Together they form a unique fingerprint.Projects
- 4 Finished
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Role of AMPK in Nutrient Sensing and in Cancer (Investigator Award Renewal)
Hardie, G. (Investigator)
1/10/17 → 31/03/24
Project: Research
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Rab Detection Initiative (Joint with Stanford School of Medicine, Max Plank Institute, Neuroscience Research Australia and Parkinsons Institute California)
Alessi, D. (Investigator) & Davies, P. (Investigator)
1/08/16 → 31/07/21
Project: Research
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AMP-Activated Protein Kinase - A Tumour Suppressor that Opposes the Metabolic Changes in Proliferating Cells (Programme Grant)
Hardie, G. (Investigator)
1/01/13 → 30/09/18
Project: Research