Cell growth effects of Epstein-Barr virus leader protein

Gordon J. Allan; Gareth J. Inman; Bruce D. Parker; David T. Rowe; Paul J. Farrell

doi:10.1099/0022-1317-73-6-1547

Cell growth effects of Epstein-Barr virus leader protein

Gordon J. Allan, Gareth J. Inman, Bruce D. Parker, David T. Rowe, Paul J. Farrell

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42 Citations (Scopus)

Abstract

B lymphoblastoid cell lines immortalized with P3HR1/633 Epstein-Barr virus (EBV), which has a deletion in the EBV nuclear antigen leader protein (EBNA-LP) gene, were transfected with a vector expressing wild-type EBNA-LP. The EBNA-LP transfectants grew out faster under G418 selection than control cells but expression of EBNA-LP made no significant difference to growth rate or saturation density of the resulting established cell lines. When the cells expressing EBNA-LP were allowed to grow to saturation and then diluted in fresh medium they underwent DNA synthesis more rapidly than control cultures.

Original language	English
Pages (from-to)	1547-1551
Number of pages	5
Journal	Journal of General Virology
Volume	73
Issue number	6
DOIs	https://doi.org/10.1099/0022-1317-73-6-1547
Publication status	Published - 1992

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title = "Cell growth effects of Epstein-Barr virus leader protein",

abstract = "B lymphoblastoid cell lines immortalized with P3HR1/633 Epstein-Barr virus (EBV), which has a deletion in the EBV nuclear antigen leader protein (EBNA-LP) gene, were transfected with a vector expressing wild-type EBNA-LP. The EBNA-LP transfectants grew out faster under G418 selection than control cells but expression of EBNA-LP made no significant difference to growth rate or saturation density of the resulting established cell lines. When the cells expressing EBNA-LP were allowed to grow to saturation and then diluted in fresh medium they underwent DNA synthesis more rapidly than control cultures.",

author = "Allan, {Gordon J.} and Inman, {Gareth J.} and Parker, {Bruce D.} and Rowe, {David T.} and Farrell, {Paul J.}",

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T1 - Cell growth effects of Epstein-Barr virus leader protein

AU - Allan, Gordon J.

AU - Inman, Gareth J.

AU - Parker, Bruce D.

AU - Rowe, David T.

AU - Farrell, Paul J.

PY - 1992

Y1 - 1992

N2 - B lymphoblastoid cell lines immortalized with P3HR1/633 Epstein-Barr virus (EBV), which has a deletion in the EBV nuclear antigen leader protein (EBNA-LP) gene, were transfected with a vector expressing wild-type EBNA-LP. The EBNA-LP transfectants grew out faster under G418 selection than control cells but expression of EBNA-LP made no significant difference to growth rate or saturation density of the resulting established cell lines. When the cells expressing EBNA-LP were allowed to grow to saturation and then diluted in fresh medium they underwent DNA synthesis more rapidly than control cultures.

AB - B lymphoblastoid cell lines immortalized with P3HR1/633 Epstein-Barr virus (EBV), which has a deletion in the EBV nuclear antigen leader protein (EBNA-LP) gene, were transfected with a vector expressing wild-type EBNA-LP. The EBNA-LP transfectants grew out faster under G418 selection than control cells but expression of EBNA-LP made no significant difference to growth rate or saturation density of the resulting established cell lines. When the cells expressing EBNA-LP were allowed to grow to saturation and then diluted in fresh medium they underwent DNA synthesis more rapidly than control cultures.

U2 - 10.1099/0022-1317-73-6-1547

DO - 10.1099/0022-1317-73-6-1547

M3 - Article

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SN - 0022-1317

VL - 73

SP - 1547

EP - 1551

JO - Journal of General Virology

JF - Journal of General Virology

IS - 6

ER -

Cell growth effects of Epstein-Barr virus leader protein

Abstract

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