Cell growth effects of Epstein-Barr virus leader protein

TY - JOUR

T1 - Cell growth effects of Epstein-Barr virus leader protein

AU - Allan, Gordon J.

AU - Inman, Gareth J.

AU - Parker, Bruce D.

AU - Rowe, David T.

AU - Farrell, Paul J.

PY - 1992

Y1 - 1992

N2 - B lymphoblastoid cell lines immortalized with P3HR1/633 Epstein-Barr virus (EBV), which has a deletion in the EBV nuclear antigen leader protein (EBNA-LP) gene, were transfected with a vector expressing wild-type EBNA-LP. The EBNA-LP transfectants grew out faster under G418 selection than control cells but expression of EBNA-LP made no significant difference to growth rate or saturation density of the resulting established cell lines. When the cells expressing EBNA-LP were allowed to grow to saturation and then diluted in fresh medium they underwent DNA synthesis more rapidly than control cultures.

AB - B lymphoblastoid cell lines immortalized with P3HR1/633 Epstein-Barr virus (EBV), which has a deletion in the EBV nuclear antigen leader protein (EBNA-LP) gene, were transfected with a vector expressing wild-type EBNA-LP. The EBNA-LP transfectants grew out faster under G418 selection than control cells but expression of EBNA-LP made no significant difference to growth rate or saturation density of the resulting established cell lines. When the cells expressing EBNA-LP were allowed to grow to saturation and then diluted in fresh medium they underwent DNA synthesis more rapidly than control cultures.

U2 - 10.1099/0022-1317-73-6-1547

DO - 10.1099/0022-1317-73-6-1547

M3 - Article

C2 - 1318948

SN - 0022-1317

VL - 73

SP - 1547

EP - 1551

JO - Journal of General Virology

JF - Journal of General Virology

IS - 6

ER -