Cell surface antigens of Trypanosoma cruzi: Use of monoclonal antibodies to identify and isolate an epimastigote specific glycoprotein

David Snary, Michael A. J. Ferguson, Martin T. Scott, Anthony K. Allen

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    68 Citations (Scopus)

    Abstract

    A monoclonal antibody was produced by cell fusion from mice immunised with Trypanosoma cruzi epimastigotes. The antibody was epimastigote specific but not strain specific; the antibody bound to Y, Peru and Tulahuén epimastigotes but did not bind to Y amastigotes or trypomastigotes. The antigen recognised by the monoclonal antibody was a 72 000 molecular weight cell surface glycoprotein which represented only 0.04% of the whole cell protein. Analysis of the glycoprotein purified by antibody affinity chromatography revealed a carbohydrate content of 52% by weight which was composed of glucosamine, mannose, galactose, glucose and three different pentoses: fucose, xylose and ribose. Immunisation with the purified glycoprotein did not protect mice from a lethal infection of T. cruzi.

    Original languageEnglish
    Pages (from-to)343-356
    Number of pages14
    JournalMolecular and Biochemical Parasitology
    Volume3
    Issue number6
    DOIs
    Publication statusPublished - Oct 1981

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