Cell surface antigens of Trypanosoma cruzi: Use of monoclonal antibodies to identify and isolate an epimastigote specific glycoprotein

TY - JOUR

T1 - Cell surface antigens of Trypanosoma cruzi

T2 - Use of monoclonal antibodies to identify and isolate an epimastigote specific glycoprotein

AU - Snary, David

AU - Ferguson, Michael A. J.

AU - Scott, Martin T.

AU - Allen, Anthony K.

N1 - Copyright 2008 Elsevier B.V., All rights reserved.

PY - 1981/10

Y1 - 1981/10

N2 - A monoclonal antibody was produced by cell fusion from mice immunised with Trypanosoma cruzi epimastigotes. The antibody was epimastigote specific but not strain specific; the antibody bound to Y, Peru and Tulahuén epimastigotes but did not bind to Y amastigotes or trypomastigotes. The antigen recognised by the monoclonal antibody was a 72 000 molecular weight cell surface glycoprotein which represented only 0.04% of the whole cell protein. Analysis of the glycoprotein purified by antibody affinity chromatography revealed a carbohydrate content of 52% by weight which was composed of glucosamine, mannose, galactose, glucose and three different pentoses: fucose, xylose and ribose. Immunisation with the purified glycoprotein did not protect mice from a lethal infection of T. cruzi.

AB - A monoclonal antibody was produced by cell fusion from mice immunised with Trypanosoma cruzi epimastigotes. The antibody was epimastigote specific but not strain specific; the antibody bound to Y, Peru and Tulahuén epimastigotes but did not bind to Y amastigotes or trypomastigotes. The antigen recognised by the monoclonal antibody was a 72 000 molecular weight cell surface glycoprotein which represented only 0.04% of the whole cell protein. Analysis of the glycoprotein purified by antibody affinity chromatography revealed a carbohydrate content of 52% by weight which was composed of glucosamine, mannose, galactose, glucose and three different pentoses: fucose, xylose and ribose. Immunisation with the purified glycoprotein did not protect mice from a lethal infection of T. cruzi.

UR - https://www.scopus.com/pages/publications/0019630344

U2 - 10.1016/0166-6851(81)90035-9

DO - 10.1016/0166-6851(81)90035-9

M3 - Article

AN - SCOPUS:0019630344

SN - 0166-6851

VL - 3

SP - 343

EP - 356

JO - Molecular and Biochemical Parasitology

JF - Molecular and Biochemical Parasitology

IS - 6

ER -