Cell target genes of Epstein-Barr virus transcription factor EBNA-2: induction of the p55alpha regulatory subunit of PI3-kinase and its role in survival of EREB2.5 cells

Lindsay C Spender, Walter Lucchesi, Gustavo Bodelon, Antonio Bilancio, Claudio Elgueta Karstegl, Tomoichiro Asano, Oliver Dittrich-Breiholz, Michael Kracht, Bart Vanhaesebroeck, Paul J Farrell (Lead / Corresponding author)

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    40 Citations (Scopus)

    Abstract

    Microarray analysis covering most of the annotated RNAs in the human genome identified a panel of genes induced by the Epstein-Barr virus (EBV) EBNA-2 transcription factor in the EREB2.5 human B-lymphoblastoid cell line without the need for any intermediate protein synthesis. Previous data indicating that PIK3R1 RNA (the alpha regulatory subunit of PI3-kinase) was induced were confirmed, but it is now shown that it is the p55alpha regulatory subunit that is induced. Several EBV-immortalized lymphoblastoid cell lines were shown to express p55alpha. Expression of PI3-kinase p85 regulatory and p110 catalytic subunits was not regulated by EBNA-2. Proliferation of EREB2.5 lymphoblastoid cells was inhibited by RNAi knock-down of p55alpha protein expression, loss of p55alpha being accompanied by an increase in apoptosis. p55alpha is thus a functional target of EBNA2 in EREB2.5 cells and the specific regulation of p55alpha by EBV will provide an opportunity to investigate the physiological function of p55alpha in this human cell line.

    Original languageEnglish
    Pages (from-to)2859-2867
    Number of pages9
    JournalJournal of General Virology
    Volume87
    Issue number10
    Early online date1 Oct 2006
    DOIs
    Publication statusPublished - Oct 2006

    Keywords

    • Apoptosis
    • Cell Line
    • Cell Survival
    • Enzyme Induction
    • Epstein-Barr Virus Nuclear Antigens
    • Gene Expression Profiling
    • Gene Expression Regulation
    • Herpesvirus 4, Human
    • Humans
    • Oligonucleotide Array Sequence Analysis
    • Phosphatidylinositol 3-Kinases
    • Protein Subunits
    • RNA Interference
    • Transcription Factors
    • Viral Proteins
    • Journal Article
    • Research Support, Non-U.S. Gov't

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