Cell-to-Cell Connectivity Assays for the Analysis of Cytoskeletal and Other Regulators of Plasmodesmata

Zoe Barr, Jens Tilsner (Lead / Corresponding author)

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

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Abstract

The actin cytoskeleton has close but so far incompletely understood connections to plasmodesmata, the cell junctions of plants. Plasmodesmata are essential for plant development and responses to biotic and abiotic stresses and facilitate the intercellular exchange of metabolites and hormones but also macromolecules such as proteins and RNAs. The molecular size exclusion limited of plasmodesmata is dynamically regulated, including by actin-associated proteins. Therefore, experimental analysis of plasmodesmal regulation can be relevant to plant cytoskeleton research. This chapter presents two simple imaging-based protocols for analyzing macromolecular cell-to-cell connectivity in leaves.

Original languageEnglish
Title of host publicationThe Plant Cytoskeleton
Subtitle of host publicationMethods and Protocols
EditorsPatrick J. Hussey, Pengwei Wang
Place of PublicationNew York
PublisherHumana Press Inc.
Pages193-202
Number of pages10
ISBN (Electronic)9781071628676
ISBN (Print)9781071628669 (hbk), 9781071628690 (pbk)
DOIs
Publication statusPublished - 2023

Publication series

NameMethods in Molecular Biology
Volume2604
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Acto-myosin
  • Arabidopsis thaliana
  • Microprojectile bombardment
  • Nicotiana benthamiana
  • Plasmodesmata
  • Size exclusion limit

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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