Abstract
We provide a detailed description of mesenchymal stem cells (MSCs) isolated from human periapical cysts, which we have termed hPCy-MSCs. These cells have a fibroblast-like shape and adhere to tissue culture plastic surfaces. hPCy-MSCs possess high proliferative potential and self-renewal capacity properties. We characterised the immunophenotype of hPCy-MSCs (CD73+, CD90+, CD105+, CD13+, CD29+, CD44+, CD45-, STRO-1+, CD146+) by flow cytometry and immunofluorescence. hPCy-MSCs possess the potential to differentiate into osteoblast- and adipocyte-like cells in vitro. Multi-potentiality was evaluated with culture-specific staining and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis for osteo/odontogenic and adipogenic markers. This is the first report to indicate that human periapical cysts contain cells with MSC-like properties. Taken together, our findings indicate that human periapical cysts could be a rich source of MSCs.
Original language | English |
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Pages (from-to) | 1070-1078 |
Number of pages | 9 |
Journal | International Journal of Biological Sciences |
Volume | 9 |
Issue number | 10 |
DOIs | |
Publication status | Published - 16 Nov 2013 |
Keywords
- Adipogenic differentiation
- Cyst
- Mesenchymal stem cells (MSCs)
- Mesenchymal stem cells isolated from human periapical cyst (hPCy-MSCs)
- Odontogenic cyst
- Osteogenic differentiation
- Periapical inflammatory tissues
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Applied Microbiology and Biotechnology
- Molecular Biology
- Developmental Biology
- Cell Biology