Cells isolated from human periapical cysts express mesenchymal stem cell-like properties

Massimo Marrelli, Francesco Paduano, Marco Tatullo

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62 Citations (Scopus)
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Abstract

We provide a detailed description of mesenchymal stem cells (MSCs) isolated from human periapical cysts, which we have termed hPCy-MSCs. These cells have a fibroblast-like shape and adhere to tissue culture plastic surfaces. hPCy-MSCs possess high proliferative potential and self-renewal capacity properties. We characterised the immunophenotype of hPCy-MSCs (CD73+, CD90+, CD105+, CD13+, CD29+, CD44+, CD45-, STRO-1+, CD146+) by flow cytometry and immunofluorescence. hPCy-MSCs possess the potential to differentiate into osteoblast- and adipocyte-like cells in vitro. Multi-potentiality was evaluated with culture-specific staining and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis for osteo/odontogenic and adipogenic markers. This is the first report to indicate that human periapical cysts contain cells with MSC-like properties. Taken together, our findings indicate that human periapical cysts could be a rich source of MSCs.

Original languageEnglish
Pages (from-to)1070-1078
Number of pages9
JournalInternational Journal of Biological Sciences
Volume9
Issue number10
DOIs
Publication statusPublished - 16 Nov 2013

Keywords

  • Adipogenic differentiation
  • Cyst
  • Mesenchymal stem cells (MSCs)
  • Mesenchymal stem cells isolated from human periapical cyst (hPCy-MSCs)
  • Odontogenic cyst
  • Osteogenic differentiation
  • Periapical inflammatory tissues

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