Cellular heterogeneity and drug resistance in two ovarian adenocarcinoma cell lines derived from a single patient

TY - JOUR

T1 - Cellular heterogeneity and drug resistance in two ovarian adenocarcinoma cell lines derived from a single patient

AU - Wolf, C. Roland

AU - Hayward, Ian P.

AU - Lawrie, Sandra S.

AU - Buckton, Karin

AU - McIntyre, Margaret A.

AU - Adams, David J.

AU - Lewis, Alexander D.

AU - Scott, Angela R.R.

AU - Smyth, John F.

PY - 1987/6/15

Y1 - 1987/6/15

N2 - Two ovarian cell lines were derived from the ascites of a patient before and after the onset of resistance to chemotherapy involving cis‐platinum, chlorambucil and 5‐fluorouracil. Characterization of these lines shows them to have various features in common and some significant differences. Cytologically the lines cannot be distinguished and they both contain high concentrations of oestrogen receptor. However, they do differ with respect to their growth characteristics, karyotype, glutathione content and sensitivity to cis‐platinum. The karyotypes of the 2 lines show several marker chromosomes in common but the resistant line contained a chromosome 8 and a 17 which were absent from the earlier sensitive line. This suggests a clonal origin with subsequent divergence to a heterogeneous population.

AB - Two ovarian cell lines were derived from the ascites of a patient before and after the onset of resistance to chemotherapy involving cis‐platinum, chlorambucil and 5‐fluorouracil. Characterization of these lines shows them to have various features in common and some significant differences. Cytologically the lines cannot be distinguished and they both contain high concentrations of oestrogen receptor. However, they do differ with respect to their growth characteristics, karyotype, glutathione content and sensitivity to cis‐platinum. The karyotypes of the 2 lines show several marker chromosomes in common but the resistant line contained a chromosome 8 and a 17 which were absent from the earlier sensitive line. This suggests a clonal origin with subsequent divergence to a heterogeneous population.

UR - https://www.scopus.com/pages/publications/0023218482

U2 - 10.1002/ijc.2910390607

DO - 10.1002/ijc.2910390607

M3 - Article

C2 - 3583449

AN - SCOPUS:0023218482

SN - 0020-7136

VL - 39

SP - 695

EP - 702

JO - International Journal of Cancer

JF - International Journal of Cancer

IS - 6

ER -