Cellular IP6 Levels Limit HIV Production while Viruses that Cannot Efficiently Package IP6 Are Attenuated for Infection and Replication

Donna L. Mallery, K. M.Rifat Faysal, Alex Kleinpeter, Miranda S.C. Wilson, Marina Vaysburd, Adam J. Fletcher, Mariia Novikova, Till Böcking, Eric O. Freed, Adolfo Saiardi, Leo C. James (Lead / Corresponding author)

Research output: Contribution to journalArticle

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Abstract

HIV-1 hijacks host proteins to promote infection. Here we show that HIV is also dependent upon the host metabolite inositol hexakisphosphate (IP 6) for viral production and primary cell replication. HIV-1 recruits IP 6 into virions using two lysine rings in its immature hexamers. Mutation of either ring inhibits IP 6 packaging and reduces viral production. Loss of IP 6 also results in virions with highly unstable capsids, leading to a profound loss of reverse transcription and cell infection. Replacement of one ring with a hydrophobic isoleucine core restores viral production, but IP 6 incorporation and infection remain impaired, consistent with an independent role for IP 6 in stable capsid assembly. Genetic knockout of biosynthetic kinases IPMK and IPPK reveals that cellular IP 6 availability limits the production of diverse lentiviruses, but in the absence of IP 6, HIV-1 packages IP 5 without loss of infectivity. Together, these data suggest that IP 6 is a critical cofactor for HIV-1 replication.

Original languageEnglish
Pages (from-to)3983-3996.e4
Number of pages19
JournalCell Reports
Volume29
Issue number12
Early online date17 Dec 2019
DOIs
Publication statusPublished - 17 Dec 2019

Fingerprint

Viruses
HIV-1
HIV
Capsid
Infection
Virion
Virus Assembly
Lentivirus
Phytic Acid
Isoleucine
Transcription
Metabolites
Lysine
Reverse Transcription
Packaging
Phosphotransferases
Availability
Mutation
Proteins

Keywords

  • AIDS
  • capsid
  • HIV
  • inositol hexakisphosphate
  • IP6
  • IPMK
  • IPPK
  • virus

Cite this

Mallery, Donna L. ; Faysal, K. M.Rifat ; Kleinpeter, Alex ; Wilson, Miranda S.C. ; Vaysburd, Marina ; Fletcher, Adam J. ; Novikova, Mariia ; Böcking, Till ; Freed, Eric O. ; Saiardi, Adolfo ; James, Leo C. / Cellular IP6 Levels Limit HIV Production while Viruses that Cannot Efficiently Package IP6 Are Attenuated for Infection and Replication. In: Cell Reports. 2019 ; Vol. 29, No. 12. pp. 3983-3996.e4.
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abstract = "HIV-1 hijacks host proteins to promote infection. Here we show that HIV is also dependent upon the host metabolite inositol hexakisphosphate (IP 6) for viral production and primary cell replication. HIV-1 recruits IP 6 into virions using two lysine rings in its immature hexamers. Mutation of either ring inhibits IP 6 packaging and reduces viral production. Loss of IP 6 also results in virions with highly unstable capsids, leading to a profound loss of reverse transcription and cell infection. Replacement of one ring with a hydrophobic isoleucine core restores viral production, but IP 6 incorporation and infection remain impaired, consistent with an independent role for IP 6 in stable capsid assembly. Genetic knockout of biosynthetic kinases IPMK and IPPK reveals that cellular IP 6 availability limits the production of diverse lentiviruses, but in the absence of IP 6, HIV-1 packages IP 5 without loss of infectivity. Together, these data suggest that IP 6 is a critical cofactor for HIV-1 replication.",
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Mallery, DL, Faysal, KMR, Kleinpeter, A, Wilson, MSC, Vaysburd, M, Fletcher, AJ, Novikova, M, Böcking, T, Freed, EO, Saiardi, A & James, LC 2019, 'Cellular IP6 Levels Limit HIV Production while Viruses that Cannot Efficiently Package IP6 Are Attenuated for Infection and Replication', Cell Reports, vol. 29, no. 12, pp. 3983-3996.e4. https://doi.org/10.1016/j.celrep.2019.11.050

Cellular IP6 Levels Limit HIV Production while Viruses that Cannot Efficiently Package IP6 Are Attenuated for Infection and Replication. / Mallery, Donna L.; Faysal, K. M.Rifat; Kleinpeter, Alex; Wilson, Miranda S.C.; Vaysburd, Marina; Fletcher, Adam J.; Novikova, Mariia; Böcking, Till; Freed, Eric O.; Saiardi, Adolfo; James, Leo C. (Lead / Corresponding author).

In: Cell Reports, Vol. 29, No. 12, 17.12.2019, p. 3983-3996.e4.

Research output: Contribution to journalArticle

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N2 - HIV-1 hijacks host proteins to promote infection. Here we show that HIV is also dependent upon the host metabolite inositol hexakisphosphate (IP 6) for viral production and primary cell replication. HIV-1 recruits IP 6 into virions using two lysine rings in its immature hexamers. Mutation of either ring inhibits IP 6 packaging and reduces viral production. Loss of IP 6 also results in virions with highly unstable capsids, leading to a profound loss of reverse transcription and cell infection. Replacement of one ring with a hydrophobic isoleucine core restores viral production, but IP 6 incorporation and infection remain impaired, consistent with an independent role for IP 6 in stable capsid assembly. Genetic knockout of biosynthetic kinases IPMK and IPPK reveals that cellular IP 6 availability limits the production of diverse lentiviruses, but in the absence of IP 6, HIV-1 packages IP 5 without loss of infectivity. Together, these data suggest that IP 6 is a critical cofactor for HIV-1 replication.

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