Cellular protein extraction and Western blotting using dry transfer (iBlot system): Protocol

Research output: Other contribution

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Abstract

A generic protein extraction and Western blotting protocol, allowing both for total cell lysis or cytoplasmic protein extraction only. The cytoplasmic extraction version has been used when Western blotting for PI3K and MAPK/ERK signalling components; total cell lysis with sonication has been used when Western blotting for histones.
Original languageEnglish
TypeResearch Protocol
Publisherprotocols.io
DOIs
Publication statusPublished - 26 Jun 2019

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