Cellular protein extraction and Western blotting using dry transfer (iBlot system): Protocol

Ralitsa Madsen

doi:10.17504/protocols.io.4r4gv8w

Cellular protein extraction and Western blotting using dry transfer (iBlot system): Protocol

Ralitsa Madsen

MRC PPU

Research output: Other contribution

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Abstract

A generic protein extraction and Western blotting protocol, allowing both for total cell lysis or cytoplasmic protein extraction only. The cytoplasmic extraction version has been used when Western blotting for PI3K and MAPK/ERK signalling components; total cell lysis with sonication has been used when Western blotting for histones.

Original language	English
Type	Research Protocol
Publisher	protocols.io
DOIs	https://doi.org/10.17504/protocols.io.4r4gv8w
Publication status	Published - 26 Jun 2019

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10.17504/protocols.io.4r4gv8wLicence: CC BY

Final Published VersionFinal published version, 449 KBLicence: CC BY

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title = "Cellular protein extraction and Western blotting using dry transfer (iBlot system): Protocol",

abstract = "A generic protein extraction and Western blotting protocol, allowing both for total cell lysis or cytoplasmic protein extraction only. The cytoplasmic extraction version has been used when Western blotting for PI3K and MAPK/ERK signalling components; total cell lysis with sonication has been used when Western blotting for histones.",

author = "Ralitsa Madsen",

note = "Wellcome Trust Grant ID: 220464/Z/20/Z",

year = "2019",

month = jun,

day = "26",

doi = "10.17504/protocols.io.4r4gv8w",

language = "English",

publisher = "protocols.io",

type = "Other",

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T2 - Protocol

AU - Madsen, Ralitsa

N1 - Wellcome Trust Grant ID: 220464/Z/20/Z

PY - 2019/6/26

Y1 - 2019/6/26

N2 - A generic protein extraction and Western blotting protocol, allowing both for total cell lysis or cytoplasmic protein extraction only. The cytoplasmic extraction version has been used when Western blotting for PI3K and MAPK/ERK signalling components; total cell lysis with sonication has been used when Western blotting for histones.

AB - A generic protein extraction and Western blotting protocol, allowing both for total cell lysis or cytoplasmic protein extraction only. The cytoplasmic extraction version has been used when Western blotting for PI3K and MAPK/ERK signalling components; total cell lysis with sonication has been used when Western blotting for histones.

U2 - 10.17504/protocols.io.4r4gv8w

DO - 10.17504/protocols.io.4r4gv8w

M3 - Other contribution

PB - protocols.io

ER -

Cellular protein extraction and Western blotting using dry transfer (iBlot system): Protocol

Abstract

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