CENP-A confers a reduction in height on octameric nucleosomes

Matthew D.D. Miell, Helena M. Barysz, Juri Rappsilber, Robin C. Allshire, Colin J. Fuller, Aaron F. Straight, Annika Guse, Andrew Downes, Tom Owen-Hughes

    Research output: Contribution to journalArticle

    35 Citations (Scopus)

    Abstract

    Nucleosomes with histone H3 replaced by CENP-A direct kinetochore assembly. CENP-A nucleosomes from human and Drosophila have been reported to have reduced heights as compared to canonical octameric H3 nucleosomes, thus suggesting a unique tetrameric hemisomal composition. We demonstrate that octameric CENP-A nucleosomes assembled in vitro exhibit reduced heights, indicating that they are physically distinct from H3 nucleosomes and negating the need to invoke the presence of hemisomes.
    Original languageEnglish
    JournalNature Structural & Molecular Biology
    Early online date5 May 2013
    DOIs
    Publication statusPublished - 2013

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    Nucleosomes
    Kinetochores
    Histones
    Drosophila
    centromere protein A

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    Miell, M. D. D., Barysz, H. M., Rappsilber, J., Allshire, R. C., Fuller, C. J., Straight, A. F., ... Owen-Hughes, T. (2013). CENP-A confers a reduction in height on octameric nucleosomes. Nature Structural & Molecular Biology. https://doi.org/10.1038/nsmb.2574
    Miell, Matthew D.D. ; Barysz, Helena M. ; Rappsilber, Juri ; Allshire, Robin C. ; Fuller, Colin J. ; Straight, Aaron F. ; Guse, Annika ; Downes, Andrew ; Owen-Hughes, Tom. / CENP-A confers a reduction in height on octameric nucleosomes. In: Nature Structural & Molecular Biology. 2013.
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    Miell, MDD, Barysz, HM, Rappsilber, J, Allshire, RC, Fuller, CJ, Straight, AF, Guse, A, Downes, A & Owen-Hughes, T 2013, 'CENP-A confers a reduction in height on octameric nucleosomes', Nature Structural & Molecular Biology. https://doi.org/10.1038/nsmb.2574

    CENP-A confers a reduction in height on octameric nucleosomes. / Miell, Matthew D.D.; Barysz, Helena M.; Rappsilber, Juri; Allshire, Robin C.; Fuller, Colin J.; Straight, Aaron F.; Guse, Annika; Downes, Andrew; Owen-Hughes, Tom.

    In: Nature Structural & Molecular Biology, 2013.

    Research output: Contribution to journalArticle

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    AU - Barysz, Helena M.

    AU - Rappsilber, Juri

    AU - Allshire, Robin C.

    AU - Fuller, Colin J.

    AU - Straight, Aaron F.

    AU - Guse, Annika

    AU - Downes, Andrew

    AU - Owen-Hughes, Tom

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    AB - Nucleosomes with histone H3 replaced by CENP-A direct kinetochore assembly. CENP-A nucleosomes from human and Drosophila have been reported to have reduced heights as compared to canonical octameric H3 nucleosomes, thus suggesting a unique tetrameric hemisomal composition. We demonstrate that octameric CENP-A nucleosomes assembled in vitro exhibit reduced heights, indicating that they are physically distinct from H3 nucleosomes and negating the need to invoke the presence of hemisomes.

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    Miell MDD, Barysz HM, Rappsilber J, Allshire RC, Fuller CJ, Straight AF et al. CENP-A confers a reduction in height on octameric nucleosomes. Nature Structural & Molecular Biology. 2013. https://doi.org/10.1038/nsmb.2574