CENP-A confers a reduction in height on octameric nucleosomes

Matthew D.D. Miell; Helena M. Barysz; Juri Rappsilber; Robin C. Allshire; Colin J. Fuller; Aaron F. Straight; Annika Guse; Andrew Downes; Tom Owen-Hughes

doi:10.1038/nsmb.2574

CENP-A confers a reduction in height on octameric nucleosomes

Matthew D.D. Miell, Helena M. Barysz, Juri Rappsilber, Robin C. Allshire, Colin J. Fuller, Aaron F. Straight, Annika Guse, Andrew Downes, Tom Owen-Hughes

Research output: Contribution to journal › Article

37 Citations (Scopus)

Abstract

Nucleosomes with histone H3 replaced by CENP-A direct kinetochore assembly. CENP-A nucleosomes from human and Drosophila have been reported to have reduced heights as compared to canonical octameric H3 nucleosomes, thus suggesting a unique tetrameric hemisomal composition. We demonstrate that octameric CENP-A nucleosomes assembled in vitro exhibit reduced heights, indicating that they are physically distinct from H3 nucleosomes and negating the need to invoke the presence of hemisomes.

Original language	English
Journal	Nature Structural & Molecular Biology
Early online date	5 May 2013
DOIs	https://doi.org/10.1038/nsmb.2574
Publication status	Published - 2013

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Nucleosomes

Kinetochores

Histones

Drosophila

centromere protein A

Cite this

Miell, M. D. D., Barysz, H. M., Rappsilber, J., Allshire, R. C., Fuller, C. J., Straight, A. F., ... Owen-Hughes, T. (2013). CENP-A confers a reduction in height on octameric nucleosomes. Nature Structural & Molecular Biology. https://doi.org/10.1038/nsmb.2574

Miell, Matthew D.D. ; Barysz, Helena M. ; Rappsilber, Juri ; Allshire, Robin C. ; Fuller, Colin J. ; Straight, Aaron F. ; Guse, Annika ; Downes, Andrew ; Owen-Hughes, Tom. / CENP-A confers a reduction in height on octameric nucleosomes. In: Nature Structural & Molecular Biology. 2013.

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abstract = "Nucleosomes with histone H3 replaced by CENP-A direct kinetochore assembly. CENP-A nucleosomes from human and Drosophila have been reported to have reduced heights as compared to canonical octameric H3 nucleosomes, thus suggesting a unique tetrameric hemisomal composition. We demonstrate that octameric CENP-A nucleosomes assembled in vitro exhibit reduced heights, indicating that they are physically distinct from H3 nucleosomes and negating the need to invoke the presence of hemisomes.",

author = "Miell, {Matthew D.D.} and Barysz, {Helena M.} and Juri Rappsilber and Allshire, {Robin C.} and Fuller, {Colin J.} and Straight, {Aaron F.} and Annika Guse and Andrew Downes and Tom Owen-Hughes",

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Miell, MDD, Barysz, HM, Rappsilber, J, Allshire, RC, Fuller, CJ, Straight, AF, Guse, A, Downes, A & Owen-Hughes, T 2013, 'CENP-A confers a reduction in height on octameric nucleosomes', Nature Structural & Molecular Biology. https://doi.org/10.1038/nsmb.2574

CENP-A confers a reduction in height on octameric nucleosomes. / Miell, Matthew D.D.; Barysz, Helena M.; Rappsilber, Juri; Allshire, Robin C.; Fuller, Colin J.; Straight, Aaron F.; Guse, Annika; Downes, Andrew; Owen-Hughes, Tom.

In: Nature Structural & Molecular Biology, 2013.

Research output: Contribution to journal › Article

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AB - Nucleosomes with histone H3 replaced by CENP-A direct kinetochore assembly. CENP-A nucleosomes from human and Drosophila have been reported to have reduced heights as compared to canonical octameric H3 nucleosomes, thus suggesting a unique tetrameric hemisomal composition. We demonstrate that octameric CENP-A nucleosomes assembled in vitro exhibit reduced heights, indicating that they are physically distinct from H3 nucleosomes and negating the need to invoke the presence of hemisomes.

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Miell MDD, Barysz HM, Rappsilber J, Allshire RC, Fuller CJ, Straight AF et al. CENP-A confers a reduction in height on octameric nucleosomes. Nature Structural & Molecular Biology. 2013. https://doi.org/10.1038/nsmb.2574