CENP-A confers a reduction in height on octameric nucleosomes

Matthew D.D. Miell; Helena M. Barysz; Juri Rappsilber; Robin C. Allshire; Colin J. Fuller; Aaron F. Straight; Annika Guse; Andrew Downes; Tom Owen-Hughes

doi:10.1038/nsmb.2574

CENP-A confers a reduction in height on octameric nucleosomes

Matthew D.D. Miell, Helena M. Barysz, Juri Rappsilber, Robin C. Allshire, Colin J. Fuller, Aaron F. Straight, Annika Guse, Andrew Downes, Tom Owen-Hughes

Research output: Contribution to journal › Article

38 Citations (Scopus)

Abstract

Nucleosomes with histone H3 replaced by CENP-A direct kinetochore assembly. CENP-A nucleosomes from human and Drosophila have been reported to have reduced heights as compared to canonical octameric H3 nucleosomes, thus suggesting a unique tetrameric hemisomal composition. We demonstrate that octameric CENP-A nucleosomes assembled in vitro exhibit reduced heights, indicating that they are physically distinct from H3 nucleosomes and negating the need to invoke the presence of hemisomes.

Original language	English
Journal	Nature Structural & Molecular Biology
Early online date	5 May 2013
DOIs	https://doi.org/10.1038/nsmb.2574
Publication status	Published - 2013

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10.1038/nsmb.2574

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Projects

1 Finished

Mechanisms for Remodelling Chromatin (Senior Fellowship Renewal)

Owen-Hughes, T.

Wellcome Trust

1/01/15 → 1/06/18

Project: Research

Cite this

Miell, M. D. D., Barysz, H. M., Rappsilber, J., Allshire, R. C., Fuller, C. J., Straight, A. F., Guse, A., Downes, A., & Owen-Hughes, T. (2013). CENP-A confers a reduction in height on octameric nucleosomes. Nature Structural & Molecular Biology. https://doi.org/10.1038/nsmb.2574

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AU - Downes, Andrew

AU - Owen-Hughes, Tom

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