CENP-A confers a reduction in height on octameric nucleosomes

Matthew D.D. Miell, Helena M. Barysz, Juri Rappsilber, Robin C. Allshire, Colin J. Fuller, Aaron F. Straight, Annika Guse, Andrew Downes, Tom Owen-Hughes

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    Abstract

    Nucleosomes with histone H3 replaced by CENP-A direct kinetochore assembly. CENP-A nucleosomes from human and Drosophila have been reported to have reduced heights as compared to canonical octameric H3 nucleosomes, thus suggesting a unique tetrameric hemisomal composition. We demonstrate that octameric CENP-A nucleosomes assembled in vitro exhibit reduced heights, indicating that they are physically distinct from H3 nucleosomes and negating the need to invoke the presence of hemisomes.
    Original languageEnglish
    JournalNature Structural & Molecular Biology
    Early online date5 May 2013
    DOIs
    Publication statusPublished - 2013

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    Miell, M. D. D., Barysz, H. M., Rappsilber, J., Allshire, R. C., Fuller, C. J., Straight, A. F., Guse, A., Downes, A., & Owen-Hughes, T. (2013). CENP-A confers a reduction in height on octameric nucleosomes. Nature Structural & Molecular Biology. https://doi.org/10.1038/nsmb.2574