CENP-A confers a reduction in height on octameric nucleosomes

Matthew D.D. Miell; Helena M. Barysz; Juri Rappsilber; Robin C. Allshire; Colin J. Fuller; Aaron F. Straight; Annika Guse; Andrew Downes; Tom Owen-Hughes

doi:10.1038/nsmb.2574

CENP-A confers a reduction in height on octameric nucleosomes

Matthew D.D. Miell, Helena M. Barysz, Juri Rappsilber, Robin C. Allshire, Colin J. Fuller, Aaron F. Straight, Annika Guse, Andrew Downes, Tom Owen-Hughes

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

Nucleosomes with histone H3 replaced by CENP-A direct kinetochore assembly. CENP-A nucleosomes from human and Drosophila have been reported to have reduced heights as compared to canonical octameric H3 nucleosomes, thus suggesting a unique tetrameric hemisomal composition. We demonstrate that octameric CENP-A nucleosomes assembled in vitro exhibit reduced heights, indicating that they are physically distinct from H3 nucleosomes and negating the need to invoke the presence of hemisomes.

Original language	English
Journal	Nature Structural & Molecular Biology
Early online date	5 May 2013
DOIs	https://doi.org/10.1038/nsmb.2574
Publication status	Published - 2013

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10.1038/nsmb.2574

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title = "CENP-A confers a reduction in height on octameric nucleosomes",

abstract = "Nucleosomes with histone H3 replaced by CENP-A direct kinetochore assembly. CENP-A nucleosomes from human and Drosophila have been reported to have reduced heights as compared to canonical octameric H3 nucleosomes, thus suggesting a unique tetrameric hemisomal composition. We demonstrate that octameric CENP-A nucleosomes assembled in vitro exhibit reduced heights, indicating that they are physically distinct from H3 nucleosomes and negating the need to invoke the presence of hemisomes.",

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AU - Miell, Matthew D.D.

AU - Barysz, Helena M.

AU - Rappsilber, Juri

AU - Allshire, Robin C.

AU - Fuller, Colin J.

AU - Straight, Aaron F.

AU - Guse, Annika

AU - Downes, Andrew

AU - Owen-Hughes, Tom

PY - 2013

Y1 - 2013

N2 - Nucleosomes with histone H3 replaced by CENP-A direct kinetochore assembly. CENP-A nucleosomes from human and Drosophila have been reported to have reduced heights as compared to canonical octameric H3 nucleosomes, thus suggesting a unique tetrameric hemisomal composition. We demonstrate that octameric CENP-A nucleosomes assembled in vitro exhibit reduced heights, indicating that they are physically distinct from H3 nucleosomes and negating the need to invoke the presence of hemisomes.

AB - Nucleosomes with histone H3 replaced by CENP-A direct kinetochore assembly. CENP-A nucleosomes from human and Drosophila have been reported to have reduced heights as compared to canonical octameric H3 nucleosomes, thus suggesting a unique tetrameric hemisomal composition. We demonstrate that octameric CENP-A nucleosomes assembled in vitro exhibit reduced heights, indicating that they are physically distinct from H3 nucleosomes and negating the need to invoke the presence of hemisomes.

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DO - 10.1038/nsmb.2574

M3 - Article

JO - Nature Structural & Molecular Biology

JF - Nature Structural & Molecular Biology

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CENP-A confers a reduction in height on octameric nucleosomes

Abstract

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Strategic Award: Wellcome Trust Technology Platform

Mechanisms for Remodelling Chromatin (Senior Fellowship Renewal)

Cite this

CENP-A confers a reduction in height on octameric nucleosomes

Abstract

Access to Document

Other files and links

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Projects

Strategic Award: Wellcome Trust Technology Platform

Mechanisms for Remodelling Chromatin (Senior Fellowship Renewal)

Cite this