Cereal variety identification using MALDI-TOF mass spectrometry SNP genotyping

J. A. Pattemore (Lead / Corresponding author), N. Rice, D. F. Marshall, R. Waugh, R. J. Henry

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Correct identification of cereal varieties is important to food quality, safety and authenticity and recently, molecular markers have been applied to cereal varietal identification. Two major factors restricting the wider application of molecular markers in crop plant genetics have been throughput and cost per data point. However, in recent years, novel molecular tools, the re-invention/re-application of mature technologies, and miniaturisation of technology has both increased throughput and significantly reduced these costs. Here we have applied Sequenom® MassARRAY® MALDI-TOF mass spectrometry using a collection of recently developed SNPs to facilitate cereal varietal identification. We used a multiplexed Sequenom® MassARRAY® mass spectrometry SNP assay targeting 45 loci to genotype a collection of Australian barley varieties. Of the 45 loci screened, 33 were informative and were used to generate a unique barcode of SNPs for each variety tested. Only one variety could not be distinguished from two others due to a high level of varietal heterogeneity. This assay format provided a flexible, cost-effective, robust and moderate throughput SNP genotyping method well suited to varietal identification and purity analysis in cereals.

Original languageEnglish
Pages (from-to)356-361
Number of pages6
JournalJournal of Cereal Science
Volume52
Issue number3
Early online date22 Jul 2010
DOIs
Publication statusPublished - 1 Nov 2010

Keywords

  • Cereal
  • MALDI-TOF
  • Single nucleotide polymorphism
  • Variety identification

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