Changes in hepatic xenobiotic-metabolising enzymes in mouse liver following infection with Leishmania donovani

Graham H. Coombs, C. Roland Wolf, Valerie M. Morrison, John A. Craft (Lead / Corresponding author)

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Infection of mice with Leishmania donovani resulted in decreased activities of several liver enzymes involved in the metabolism of xenobiotics. Microsomal membranes from infected livers contained reduced amounts of cytochromes P450 and b5 and NADPH-cytochrome P450 reductase. Several cytochrome P450 isoenzymes (P450-PB1, P450-PB3, P450-PCN and P450-UT1) and P450-mediated reactions (aminopyrine demethylase, aniline hydroxylase, benzphentamine demethylase and ethoxycoumarin deethylase) were affected similarly. The metabolism of two carcinogens (nitrosodimethylamine and 7,12-dimethylbenz[a]anthracene) by liver microsomal membrane preparations was also reduced. Leishmania infection caused an increase of cytosolic epoxide hydrolase and microsomal epoxide hydrolase and NADH-cytochrome b5 reductase were unaffected. The results suggest that Leishmania-infected animals are likely to have altered responses to exogenous toxins compared to uninfected animals.

Original languageEnglish
Pages (from-to)17-24
Number of pages8
JournalMolecular and Biochemical Parasitology
Volume41
Issue number1
DOIs
Publication statusPublished - 1 Jun 1990

Keywords

  • Leishmania donovani
  • Liver
  • Mouse
  • Xenobiotic

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