Changes in hepatic xenobiotic-metabolising enzymes in mouse liver following infection with Leishmania donovani

Graham H. Coombs; C. Roland Wolf; Valerie M. Morrison; John A. Craft

doi:10.1016/0166-6851(90)90092-Z

Changes in hepatic xenobiotic-metabolising enzymes in mouse liver following infection with Leishmania donovani

Graham H. Coombs, C. Roland Wolf, Valerie M. Morrison, John A. Craft (Lead / Corresponding author)

Systems Medicine

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Infection of mice with Leishmania donovani resulted in decreased activities of several liver enzymes involved in the metabolism of xenobiotics. Microsomal membranes from infected livers contained reduced amounts of cytochromes P450 and b₅ and NADPH-cytochrome P450 reductase. Several cytochrome P450 isoenzymes (P450-PB1, P450-PB3, P450-PCN and P450-UT1) and P450-mediated reactions (aminopyrine demethylase, aniline hydroxylase, benzphentamine demethylase and ethoxycoumarin deethylase) were affected similarly. The metabolism of two carcinogens (nitrosodimethylamine and 7,12-dimethylbenz[a]anthracene) by liver microsomal membrane preparations was also reduced. Leishmania infection caused an increase of cytosolic epoxide hydrolase and microsomal epoxide hydrolase and NADH-cytochrome b₅ reductase were unaffected. The results suggest that Leishmania-infected animals are likely to have altered responses to exogenous toxins compared to uninfected animals.

Original language	English
Pages (from-to)	17-24
Number of pages	8
Journal	Molecular and Biochemical Parasitology
Volume	41
Issue number	1
DOIs	https://doi.org/10.1016/0166-6851(90)90092-Z
Publication status	Published - 1 Jun 1990

Keywords

Leishmania donovani
Liver
Mouse
Xenobiotic

ASJC Scopus subject areas

Parasitology
Molecular Biology

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10.1016/0166-6851(90)90092-Z

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title = "Changes in hepatic xenobiotic-metabolising enzymes in mouse liver following infection with Leishmania donovani",

abstract = "Infection of mice with Leishmania donovani resulted in decreased activities of several liver enzymes involved in the metabolism of xenobiotics. Microsomal membranes from infected livers contained reduced amounts of cytochromes P450 and b5 and NADPH-cytochrome P450 reductase. Several cytochrome P450 isoenzymes (P450-PB1, P450-PB3, P450-PCN and P450-UT1) and P450-mediated reactions (aminopyrine demethylase, aniline hydroxylase, benzphentamine demethylase and ethoxycoumarin deethylase) were affected similarly. The metabolism of two carcinogens (nitrosodimethylamine and 7,12-dimethylbenz[a]anthracene) by liver microsomal membrane preparations was also reduced. Leishmania infection caused an increase of cytosolic epoxide hydrolase and microsomal epoxide hydrolase and NADH-cytochrome b5 reductase were unaffected. The results suggest that Leishmania-infected animals are likely to have altered responses to exogenous toxins compared to uninfected animals.",

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author = "Coombs, {Graham H.} and {Roland Wolf}, C. and Morrison, {Valerie M.} and Craft, {John A.}",

year = "1990",

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AU - Coombs, Graham H.

AU - Roland Wolf, C.

AU - Morrison, Valerie M.

AU - Craft, John A.

PY - 1990/6/1

Y1 - 1990/6/1

N2 - Infection of mice with Leishmania donovani resulted in decreased activities of several liver enzymes involved in the metabolism of xenobiotics. Microsomal membranes from infected livers contained reduced amounts of cytochromes P450 and b5 and NADPH-cytochrome P450 reductase. Several cytochrome P450 isoenzymes (P450-PB1, P450-PB3, P450-PCN and P450-UT1) and P450-mediated reactions (aminopyrine demethylase, aniline hydroxylase, benzphentamine demethylase and ethoxycoumarin deethylase) were affected similarly. The metabolism of two carcinogens (nitrosodimethylamine and 7,12-dimethylbenz[a]anthracene) by liver microsomal membrane preparations was also reduced. Leishmania infection caused an increase of cytosolic epoxide hydrolase and microsomal epoxide hydrolase and NADH-cytochrome b5 reductase were unaffected. The results suggest that Leishmania-infected animals are likely to have altered responses to exogenous toxins compared to uninfected animals.

AB - Infection of mice with Leishmania donovani resulted in decreased activities of several liver enzymes involved in the metabolism of xenobiotics. Microsomal membranes from infected livers contained reduced amounts of cytochromes P450 and b5 and NADPH-cytochrome P450 reductase. Several cytochrome P450 isoenzymes (P450-PB1, P450-PB3, P450-PCN and P450-UT1) and P450-mediated reactions (aminopyrine demethylase, aniline hydroxylase, benzphentamine demethylase and ethoxycoumarin deethylase) were affected similarly. The metabolism of two carcinogens (nitrosodimethylamine and 7,12-dimethylbenz[a]anthracene) by liver microsomal membrane preparations was also reduced. Leishmania infection caused an increase of cytosolic epoxide hydrolase and microsomal epoxide hydrolase and NADH-cytochrome b5 reductase were unaffected. The results suggest that Leishmania-infected animals are likely to have altered responses to exogenous toxins compared to uninfected animals.

KW - Leishmania donovani

KW - Liver

KW - Mouse

KW - Xenobiotic

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VL - 41

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ER -

Changes in hepatic xenobiotic-metabolising enzymes in mouse liver following infection with Leishmania donovani

Abstract

Keywords

ASJC Scopus subject areas

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