Changes in nano-mechanical properties of human epidermal cornified cells in children with atopic dermatitis

Marek Haftek, Maeve A. McAleer, Ivone Jakasa, W. H. Irwin McLean, Sanja Kezic, Alan D. Irvine (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    17 Downloads (Pure)

    Abstract

    Background: Impaired skin barrier is an important etiological factor in atopic dermatitis (AD). The structural protein filaggrin (FLG) plays a major role in maintenance of the competent skin barrier and its deficiency is associated with enhanced susceptibility to mechanical injury. Here we examined biomechanical characteristics of the corneocytes in children with AD and healthy controls. Methods: We recruited 20 children with AD and 7 healthy children. They were genotyped for filaggrin gene ( FLG) loss-of-function mutations. Stratum corneum was collected from clinically unaffected skin by adhesive tapes. Cell stiffness (apparent elastic modulus, Ea) was determined by atomic force microscopy and filaggrin degradation products (NMF) by liquid chromatography. Skin barrier function was assessed through trans-epidermal water loss (TEWL) and disease severity by the SCORing Atopic Dermatitis (SCORAD) tool. Results: Corneocytes collected from AD patients showed a decreased elastic modulus which was strongly correlated with NMF and TEWL, but not with SCORAD. As compared with healthy controls, AD patients had reduced TEWL and NMF levels regardless of FLG mutations. NMF was strongly correlated with TEWL. Conclusion: Our findings demonstrate that AD patients have decreased corneocyte stiffness which correlates with reduced levels of filaggrin degradation products, NMF and skin barrier function. Altered mechanical properties of the corneocytes likely contribute to the loss of mechanical integrity of the SC and to reduced skin barrier function in AD.

    Original languageEnglish
    Article number97
    Number of pages19
    JournalWellcome Open Research
    Volume5
    Early online date19 May 2020
    DOIs
    Publication statusPublished - 17 Jul 2020

    Keywords

    • Atomic Force Microscopy
    • Atopic dermatitis
    • Corneocyte stiffness
    • Elastic modulus
    • Filaggrin
    • Natural Moisturizing Factor

    Fingerprint Dive into the research topics of 'Changes in nano-mechanical properties of human epidermal cornified cells in children with atopic dermatitis'. Together they form a unique fingerprint.

  • Projects

    Cite this