Characterisation of a complementary DNA encoding a novel plant enzyme with sucrolytic activity

Gordon C. Machray; L. Burch; P. E. Hedley; H. V. Davies; R. Waugh

doi:10.1016/0014-5793(94)01105-2

Characterisation of a complementary DNA encoding a novel plant enzyme with sucrolytic activity

Gordon C. Machray, L. Burch, P. E. Hedley, H. V. Davies, R. Waugh

Plant Sciences

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The cloning of a 1332 bp cDNA from a potato (Solanum tuberosumL.) cv. Cara leaf cDNA expression library, using an antibody raised against a purified tuber protein preparation with sucrolytic activity, is described. The corresponding gene in potato is of low copy number, is expressed in a variety of tissues, and encodes a protein which includes several domains with similarity to database sequences, including ferredoxin from Clostridium pasteurianum. Expression of the cDNA in E. coli yields a fusion protein with sucrolytic activity.

Original language	English
Pages (from-to)	123-127
Number of pages	5
Journal	FEBS Letters
Volume	354
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(94)01105-2
Publication status	Published - 31 Oct 1994

Keywords

Ferredoxin
Potato
Solanum tuberosum
Sucrose cleavage

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(94)01105-2

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title = "Characterisation of a complementary DNA encoding a novel plant enzyme with sucrolytic activity",

abstract = "The cloning of a 1332 bp cDNA from a potato (Solanum tuberosumL.) cv. Cara leaf cDNA expression library, using an antibody raised against a purified tuber protein preparation with sucrolytic activity, is described. The corresponding gene in potato is of low copy number, is expressed in a variety of tissues, and encodes a protein which includes several domains with similarity to database sequences, including ferredoxin from Clostridium pasteurianum. Expression of the cDNA in E. coli yields a fusion protein with sucrolytic activity.",

keywords = "Ferredoxin, Potato, Solanum tuberosum, Sucrose cleavage",

author = "Machray, {Gordon C.} and L. Burch and Hedley, {P. E.} and Davies, {H. V.} and R. Waugh",

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AU - Machray, Gordon C.

AU - Burch, L.

AU - Hedley, P. E.

AU - Davies, H. V.

AU - Waugh, R.

PY - 1994/10/31

Y1 - 1994/10/31

N2 - The cloning of a 1332 bp cDNA from a potato (Solanum tuberosumL.) cv. Cara leaf cDNA expression library, using an antibody raised against a purified tuber protein preparation with sucrolytic activity, is described. The corresponding gene in potato is of low copy number, is expressed in a variety of tissues, and encodes a protein which includes several domains with similarity to database sequences, including ferredoxin from Clostridium pasteurianum. Expression of the cDNA in E. coli yields a fusion protein with sucrolytic activity.

AB - The cloning of a 1332 bp cDNA from a potato (Solanum tuberosumL.) cv. Cara leaf cDNA expression library, using an antibody raised against a purified tuber protein preparation with sucrolytic activity, is described. The corresponding gene in potato is of low copy number, is expressed in a variety of tissues, and encodes a protein which includes several domains with similarity to database sequences, including ferredoxin from Clostridium pasteurianum. Expression of the cDNA in E. coli yields a fusion protein with sucrolytic activity.

KW - Ferredoxin

KW - Potato

KW - Solanum tuberosum

KW - Sucrose cleavage

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AN - SCOPUS:0027938925

SN - 0014-5793

VL - 354

SP - 123

EP - 127

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Characterisation of a complementary DNA encoding a novel plant enzyme with sucrolytic activity

Abstract

Keywords

ASJC Scopus subject areas

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