Characterisation of a complementary DNA encoding a novel plant enzyme with sucrolytic activity

Gordon C. Machray, L. Burch, P. E. Hedley, H. V. Davies, R. Waugh

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

The cloning of a 1332 bp cDNA from a potato (Solanum tuberosumL.) cv. Cara leaf cDNA expression library, using an antibody raised against a purified tuber protein preparation with sucrolytic activity, is described. The corresponding gene in potato is of low copy number, is expressed in a variety of tissues, and encodes a protein which includes several domains with similarity to database sequences, including ferredoxin from Clostridium pasteurianum. Expression of the cDNA in E. coli yields a fusion protein with sucrolytic activity.

Original languageEnglish
Pages (from-to)123-127
Number of pages5
JournalFEBS Letters
Volume354
Issue number1
DOIs
Publication statusPublished - 31 Oct 1994

Keywords

  • Ferredoxin
  • Potato
  • Solanum tuberosum
  • Sucrose cleavage

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