Characterisation of barley resistance to rhynchosporium on chromosome 6HS

Max Coulter, Bianca Büttner, Kerstin Hofmann, Micha Bayer, Luke Ramsay, Günther Schweizer, Robbie Waugh, Mark E. Looseley, Anna Avrova (Lead / Corresponding author)

Research output: Contribution to journalArticle

Abstract

Rhynchosporium or barley scald caused by a fungal pathogen Rhynchosporium commune is one of the most destructive and economically important diseases of barley in the world. Testing of Steptoe × Morex and CIho 3515 × Alexis doubled haploid populations has revealed a large effect QTL for resistance to R. commune close to the telomere on the short arm of chromosome 6H, present in both populations. Mapping markers flanking the QTL from both populations onto the 2017 Morex genome assembly revealed a rhynchosporium resistance locus independent of Rrs13 that we named Rrs18. The causal gene was fine mapped to an interval of 660 Kb using Steptoe × Morex backcross 1 S2 and S3 lines with molecular markers developed from Steptoe exome capture variant calling. Sequencing RNA from CIho 3515 and Alexis revealed that only 4 genes within the Rrs18 interval were transcribed in leaf tissue with a serine/threonine protein kinase being the most likely candidate for Rrs18.

Original languageEnglish
Pages (from-to)1089-1107
Number of pages19
JournalTheoretical and Applied Genetics
Volume132
Issue number4
Early online date13 Dec 2018
DOIs
Publication statusPublished - Apr 2019

Fingerprint

Rhynchosporium
Hordeum
Chromosomes
barley
chromosomes
quantitative trait loci
Population
Exome
RNA Sequence Analysis
genome assembly
Protein-Serine-Threonine Kinases
Haploidy
telomeres
Telomere
doubled haploids
Genes
genes
sequence analysis
Genome
loci

Keywords

  • barley
  • Rhynchosporium commune
  • resistance
  • QTL
  • Rrs18
  • scald

Cite this

Coulter, M., Büttner, B., Hofmann, K., Bayer, M., Ramsay, L., Schweizer, G., ... Avrova, A. (2019). Characterisation of barley resistance to rhynchosporium on chromosome 6HS. Theoretical and Applied Genetics, 132(4), 1089-1107. https://doi.org/10.1007/s00122-018-3262-8
Coulter, Max ; Büttner, Bianca ; Hofmann, Kerstin ; Bayer, Micha ; Ramsay, Luke ; Schweizer, Günther ; Waugh, Robbie ; Looseley, Mark E. ; Avrova, Anna. / Characterisation of barley resistance to rhynchosporium on chromosome 6HS. In: Theoretical and Applied Genetics. 2019 ; Vol. 132, No. 4. pp. 1089-1107.
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Coulter, M, Büttner, B, Hofmann, K, Bayer, M, Ramsay, L, Schweizer, G, Waugh, R, Looseley, ME & Avrova, A 2019, 'Characterisation of barley resistance to rhynchosporium on chromosome 6HS', Theoretical and Applied Genetics, vol. 132, no. 4, pp. 1089-1107. https://doi.org/10.1007/s00122-018-3262-8

Characterisation of barley resistance to rhynchosporium on chromosome 6HS. / Coulter, Max; Büttner, Bianca; Hofmann, Kerstin; Bayer, Micha; Ramsay, Luke; Schweizer, Günther; Waugh, Robbie; Looseley, Mark E.; Avrova, Anna (Lead / Corresponding author).

In: Theoretical and Applied Genetics, Vol. 132, No. 4, 04.2019, p. 1089-1107.

Research output: Contribution to journalArticle

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