Characterisation of early transcriptional changes involving multiple signalling pathways in the Mla13 barley interaction with powdery mildew (Blumeria graminis f. sp. hordei)

Ingo Hein, Edward I Campbell, Mary Woodhead, Peter E Hedley, Vanessa Young, Wayne L Morris, Luke Ramsay, Joerg Stockhaus, Gary D Lyon, Adrian C Newton, Paul R J Birch (Lead / Corresponding author)

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Suppression subtractive hybridisation was used to isolate 21 cDNAs ( bmi1- bmi21) up-regulated 1-5 h post-inoculation (hpi) in a barley ( Hordeum vulgare L. cv. Pallas) near-isogenic line (NIL) P11 ( Mla13) challenged with either avirulent or virulent isolates of Blumeria graminis f. sp. hordei. Transcriptional changes at these time-points are crucial for the Mla-mediated hypersensitive response [W.R. Bushnell and Z. Liu (1994) Physiol Mol Plant Pathol 44:389-402]. Seven sequences were up-regulated by 1 hpi, when the pathogen has formed only the primary germ tube. Some transcripts were similar to genes with a role in regulating programmed cell death in animals, including NF kappaB and oxysterol-binding protein. Moreover, bmi7, similar to rice resistance gene Xa21, was rapidly up-regulated in both compatible and incompatible interactions, but was then down-regulated by 5 hpi in the virulent interaction. Only nine of the transcripts were up-regulated in mlo5 resistance in cv. Pallas NIL P22, confirming differential pathway induction between Mla13 and mlo5. However, eight sequences up-regulated in the Mla13 response in P11 were already highly elevated in uninoculated mlo5 mutant P22, suggesting that they may be negatively regulated by wild-type Mlo. Regulation of bmi sequences was investigated using salicylic acid, methyl jasmonate, ethylene, H(2)O(2), abscisic acid, wounding and a glucan elicitor. No single stimulus up-regulated all genes, suggesting either combinations of these stimuli, or additional stimuli, are involved in early Mla13 and mlo5 resistances. Whereas H(2)O(2) up- or down-regulated 17 of the transcripts detected in Northern analyses, salicylic acid stimulated only down-regulation of 5 transcripts.

Original languageEnglish
Pages (from-to)803-813
Number of pages11
JournalPlanta
Volume218
Issue number5
DOIs
Publication statusPublished - Mar 2004

Fingerprint

Blumeria graminis f. sp. hordei
Hordeum
powdery mildew
Salicylic Acid
barley
isogenic lines
salicylic acid
Genes
transcription factor NF-kappa B
Abscisic Acid
suppression subtractive hybridization
genes
Glucans
NF-kappa B
germ tube
methyl jasmonate
hypersensitive response
glucans
Hordeum vulgare
abscisic acid

Keywords

  • Abscisic Acid/pharmacology
  • Acetates/pharmacology
  • Apoptosis
  • Ascomycota/growth & development
  • Blotting, Northern
  • Cyclopentanes/pharmacology
  • DNA, Complementary
  • Ethylenes/pharmacology
  • Gene Expression Regulation, Plant/drug effects
  • Glucans/pharmacology
  • Hordeum/genetics
  • Hydrogen Peroxide/pharmacology
  • Immunity, Innate/drug effects
  • Oxylipins
  • Plant Diseases/genetics
  • Plant Proteins/genetics
  • Salicylic Acid/pharmacology
  • Signal Transduction/drug effects
  • Stress, Mechanical
  • Transcriptional Activation

Cite this

Hein, Ingo ; Campbell, Edward I ; Woodhead, Mary ; Hedley, Peter E ; Young, Vanessa ; Morris, Wayne L ; Ramsay, Luke ; Stockhaus, Joerg ; Lyon, Gary D ; Newton, Adrian C ; Birch, Paul R J. / Characterisation of early transcriptional changes involving multiple signalling pathways in the Mla13 barley interaction with powdery mildew (Blumeria graminis f. sp. hordei). In: Planta. 2004 ; Vol. 218, No. 5. pp. 803-813.
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abstract = "Suppression subtractive hybridisation was used to isolate 21 cDNAs ( bmi1- bmi21) up-regulated 1-5 h post-inoculation (hpi) in a barley ( Hordeum vulgare L. cv. Pallas) near-isogenic line (NIL) P11 ( Mla13) challenged with either avirulent or virulent isolates of Blumeria graminis f. sp. hordei. Transcriptional changes at these time-points are crucial for the Mla-mediated hypersensitive response [W.R. Bushnell and Z. Liu (1994) Physiol Mol Plant Pathol 44:389-402]. Seven sequences were up-regulated by 1 hpi, when the pathogen has formed only the primary germ tube. Some transcripts were similar to genes with a role in regulating programmed cell death in animals, including NF kappaB and oxysterol-binding protein. Moreover, bmi7, similar to rice resistance gene Xa21, was rapidly up-regulated in both compatible and incompatible interactions, but was then down-regulated by 5 hpi in the virulent interaction. Only nine of the transcripts were up-regulated in mlo5 resistance in cv. Pallas NIL P22, confirming differential pathway induction between Mla13 and mlo5. However, eight sequences up-regulated in the Mla13 response in P11 were already highly elevated in uninoculated mlo5 mutant P22, suggesting that they may be negatively regulated by wild-type Mlo. Regulation of bmi sequences was investigated using salicylic acid, methyl jasmonate, ethylene, H(2)O(2), abscisic acid, wounding and a glucan elicitor. No single stimulus up-regulated all genes, suggesting either combinations of these stimuli, or additional stimuli, are involved in early Mla13 and mlo5 resistances. Whereas H(2)O(2) up- or down-regulated 17 of the transcripts detected in Northern analyses, salicylic acid stimulated only down-regulation of 5 transcripts.",
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author = "Ingo Hein and Campbell, {Edward I} and Mary Woodhead and Hedley, {Peter E} and Vanessa Young and Morris, {Wayne L} and Luke Ramsay and Joerg Stockhaus and Lyon, {Gary D} and Newton, {Adrian C} and Birch, {Paul R J}",
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Characterisation of early transcriptional changes involving multiple signalling pathways in the Mla13 barley interaction with powdery mildew (Blumeria graminis f. sp. hordei). / Hein, Ingo; Campbell, Edward I; Woodhead, Mary; Hedley, Peter E; Young, Vanessa; Morris, Wayne L; Ramsay, Luke; Stockhaus, Joerg; Lyon, Gary D; Newton, Adrian C; Birch, Paul R J (Lead / Corresponding author).

In: Planta, Vol. 218, No. 5, 03.2004, p. 803-813.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Characterisation of early transcriptional changes involving multiple signalling pathways in the Mla13 barley interaction with powdery mildew (Blumeria graminis f. sp. hordei)

AU - Hein, Ingo

AU - Campbell, Edward I

AU - Woodhead, Mary

AU - Hedley, Peter E

AU - Young, Vanessa

AU - Morris, Wayne L

AU - Ramsay, Luke

AU - Stockhaus, Joerg

AU - Lyon, Gary D

AU - Newton, Adrian C

AU - Birch, Paul R J

PY - 2004/3

Y1 - 2004/3

N2 - Suppression subtractive hybridisation was used to isolate 21 cDNAs ( bmi1- bmi21) up-regulated 1-5 h post-inoculation (hpi) in a barley ( Hordeum vulgare L. cv. Pallas) near-isogenic line (NIL) P11 ( Mla13) challenged with either avirulent or virulent isolates of Blumeria graminis f. sp. hordei. Transcriptional changes at these time-points are crucial for the Mla-mediated hypersensitive response [W.R. Bushnell and Z. Liu (1994) Physiol Mol Plant Pathol 44:389-402]. Seven sequences were up-regulated by 1 hpi, when the pathogen has formed only the primary germ tube. Some transcripts were similar to genes with a role in regulating programmed cell death in animals, including NF kappaB and oxysterol-binding protein. Moreover, bmi7, similar to rice resistance gene Xa21, was rapidly up-regulated in both compatible and incompatible interactions, but was then down-regulated by 5 hpi in the virulent interaction. Only nine of the transcripts were up-regulated in mlo5 resistance in cv. Pallas NIL P22, confirming differential pathway induction between Mla13 and mlo5. However, eight sequences up-regulated in the Mla13 response in P11 were already highly elevated in uninoculated mlo5 mutant P22, suggesting that they may be negatively regulated by wild-type Mlo. Regulation of bmi sequences was investigated using salicylic acid, methyl jasmonate, ethylene, H(2)O(2), abscisic acid, wounding and a glucan elicitor. No single stimulus up-regulated all genes, suggesting either combinations of these stimuli, or additional stimuli, are involved in early Mla13 and mlo5 resistances. Whereas H(2)O(2) up- or down-regulated 17 of the transcripts detected in Northern analyses, salicylic acid stimulated only down-regulation of 5 transcripts.

AB - Suppression subtractive hybridisation was used to isolate 21 cDNAs ( bmi1- bmi21) up-regulated 1-5 h post-inoculation (hpi) in a barley ( Hordeum vulgare L. cv. Pallas) near-isogenic line (NIL) P11 ( Mla13) challenged with either avirulent or virulent isolates of Blumeria graminis f. sp. hordei. Transcriptional changes at these time-points are crucial for the Mla-mediated hypersensitive response [W.R. Bushnell and Z. Liu (1994) Physiol Mol Plant Pathol 44:389-402]. Seven sequences were up-regulated by 1 hpi, when the pathogen has formed only the primary germ tube. Some transcripts were similar to genes with a role in regulating programmed cell death in animals, including NF kappaB and oxysterol-binding protein. Moreover, bmi7, similar to rice resistance gene Xa21, was rapidly up-regulated in both compatible and incompatible interactions, but was then down-regulated by 5 hpi in the virulent interaction. Only nine of the transcripts were up-regulated in mlo5 resistance in cv. Pallas NIL P22, confirming differential pathway induction between Mla13 and mlo5. However, eight sequences up-regulated in the Mla13 response in P11 were already highly elevated in uninoculated mlo5 mutant P22, suggesting that they may be negatively regulated by wild-type Mlo. Regulation of bmi sequences was investigated using salicylic acid, methyl jasmonate, ethylene, H(2)O(2), abscisic acid, wounding and a glucan elicitor. No single stimulus up-regulated all genes, suggesting either combinations of these stimuli, or additional stimuli, are involved in early Mla13 and mlo5 resistances. Whereas H(2)O(2) up- or down-regulated 17 of the transcripts detected in Northern analyses, salicylic acid stimulated only down-regulation of 5 transcripts.

KW - Abscisic Acid/pharmacology

KW - Acetates/pharmacology

KW - Apoptosis

KW - Ascomycota/growth & development

KW - Blotting, Northern

KW - Cyclopentanes/pharmacology

KW - DNA, Complementary

KW - Ethylenes/pharmacology

KW - Gene Expression Regulation, Plant/drug effects

KW - Glucans/pharmacology

KW - Hordeum/genetics

KW - Hydrogen Peroxide/pharmacology

KW - Immunity, Innate/drug effects

KW - Oxylipins

KW - Plant Diseases/genetics

KW - Plant Proteins/genetics

KW - Salicylic Acid/pharmacology

KW - Signal Transduction/drug effects

KW - Stress, Mechanical

KW - Transcriptional Activation

U2 - 10.1007/s00425-003-1159-4

DO - 10.1007/s00425-003-1159-4

M3 - Article

VL - 218

SP - 803

EP - 813

JO - Planta

JF - Planta

SN - 0032-0935

IS - 5

ER -