Characterisation of Phytophthora inundata according to host range, morphological variation and multigene molecular phylogeny

Banafsheh Safaiefarahani, Reza Mostowfizadeh-Ghalamfarsa, David E. L. Cooke

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    Phytophthora inundata is an Oomycetous plant pathogen that has been isolated from a range of different plant species. Although formally described in 2003, relatively little further characterisation of this taxon has been reported. This study presents an approach to phylogenetic discrimination of P. inundata based on concordance of multiple gene genealogies in relation to the host range and morphological traits of isolates from Iran (Beta vulgaris, Pistacia vera, Solanum nigrum and river water), United Kingdom (Salix matsudana), Spain (Olea sp.) and the United States of America (unknown host). Isolates were identified by rDNA internal transcribed spacer (ITS) sequence analysis, followed by assessment of pathogenicity, morphological, physiological (cultural, temperature relations and mating) and molecular traits. Multiple gene genealogy analyses were performed on regions of nuclear (ITS, ß-tubulin, translation elongation factor 1 alpha) and mitochondrial (cytochrome c oxidase subunit I) gene sequences of this and other major Phytophthora species. Congruence was observed in different phylogenetic data sets. Maximum likelihood phylogenetic reconstruction based on the different genes indicated that, although there is some variation between morphological and mating behaviour of P. inundata isolates, this taxon is a distinct and homogeneous species which always appeared as a sister taxon to P. humicola. Phytophthora inundata is shown to be a uniform species, with isolates from wide-ranging geographic origins having similar host ranges, molecular, and morphological characteristics.

    Original languageEnglish
    Pages (from-to)46-65
    Number of pages20
    JournalPhytopathologia mediterranea
    Volume52
    Issue number1
    Publication statusPublished - 2013

    Keywords

    • intraspecific variation
    • Multiple gene genealogy
    • ECOSYSTEMS
    • TREES
    • SEQUENCES
    • MEGASPERMA
    • PATHOGEN
    • AUSTRALIA
    • NORTH-AMERICA
    • MULTIPLE
    • SP NOV.

    Cite this

    Safaiefarahani, Banafsheh ; Mostowfizadeh-Ghalamfarsa, Reza ; Cooke, David E. L. / Characterisation of Phytophthora inundata according to host range, morphological variation and multigene molecular phylogeny. In: Phytopathologia mediterranea. 2013 ; Vol. 52, No. 1. pp. 46-65.
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    abstract = "Phytophthora inundata is an Oomycetous plant pathogen that has been isolated from a range of different plant species. Although formally described in 2003, relatively little further characterisation of this taxon has been reported. This study presents an approach to phylogenetic discrimination of P. inundata based on concordance of multiple gene genealogies in relation to the host range and morphological traits of isolates from Iran (Beta vulgaris, Pistacia vera, Solanum nigrum and river water), United Kingdom (Salix matsudana), Spain (Olea sp.) and the United States of America (unknown host). Isolates were identified by rDNA internal transcribed spacer (ITS) sequence analysis, followed by assessment of pathogenicity, morphological, physiological (cultural, temperature relations and mating) and molecular traits. Multiple gene genealogy analyses were performed on regions of nuclear (ITS, {\ss}-tubulin, translation elongation factor 1 alpha) and mitochondrial (cytochrome c oxidase subunit I) gene sequences of this and other major Phytophthora species. Congruence was observed in different phylogenetic data sets. Maximum likelihood phylogenetic reconstruction based on the different genes indicated that, although there is some variation between morphological and mating behaviour of P. inundata isolates, this taxon is a distinct and homogeneous species which always appeared as a sister taxon to P. humicola. Phytophthora inundata is shown to be a uniform species, with isolates from wide-ranging geographic origins having similar host ranges, molecular, and morphological characteristics.",
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    Characterisation of Phytophthora inundata according to host range, morphological variation and multigene molecular phylogeny. / Safaiefarahani, Banafsheh; Mostowfizadeh-Ghalamfarsa, Reza; Cooke, David E. L.

    In: Phytopathologia mediterranea, Vol. 52, No. 1, 2013, p. 46-65.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Characterisation of Phytophthora inundata according to host range, morphological variation and multigene molecular phylogeny

    AU - Safaiefarahani, Banafsheh

    AU - Mostowfizadeh-Ghalamfarsa, Reza

    AU - Cooke, David E. L.

    PY - 2013

    Y1 - 2013

    N2 - Phytophthora inundata is an Oomycetous plant pathogen that has been isolated from a range of different plant species. Although formally described in 2003, relatively little further characterisation of this taxon has been reported. This study presents an approach to phylogenetic discrimination of P. inundata based on concordance of multiple gene genealogies in relation to the host range and morphological traits of isolates from Iran (Beta vulgaris, Pistacia vera, Solanum nigrum and river water), United Kingdom (Salix matsudana), Spain (Olea sp.) and the United States of America (unknown host). Isolates were identified by rDNA internal transcribed spacer (ITS) sequence analysis, followed by assessment of pathogenicity, morphological, physiological (cultural, temperature relations and mating) and molecular traits. Multiple gene genealogy analyses were performed on regions of nuclear (ITS, ß-tubulin, translation elongation factor 1 alpha) and mitochondrial (cytochrome c oxidase subunit I) gene sequences of this and other major Phytophthora species. Congruence was observed in different phylogenetic data sets. Maximum likelihood phylogenetic reconstruction based on the different genes indicated that, although there is some variation between morphological and mating behaviour of P. inundata isolates, this taxon is a distinct and homogeneous species which always appeared as a sister taxon to P. humicola. Phytophthora inundata is shown to be a uniform species, with isolates from wide-ranging geographic origins having similar host ranges, molecular, and morphological characteristics.

    AB - Phytophthora inundata is an Oomycetous plant pathogen that has been isolated from a range of different plant species. Although formally described in 2003, relatively little further characterisation of this taxon has been reported. This study presents an approach to phylogenetic discrimination of P. inundata based on concordance of multiple gene genealogies in relation to the host range and morphological traits of isolates from Iran (Beta vulgaris, Pistacia vera, Solanum nigrum and river water), United Kingdom (Salix matsudana), Spain (Olea sp.) and the United States of America (unknown host). Isolates were identified by rDNA internal transcribed spacer (ITS) sequence analysis, followed by assessment of pathogenicity, morphological, physiological (cultural, temperature relations and mating) and molecular traits. Multiple gene genealogy analyses were performed on regions of nuclear (ITS, ß-tubulin, translation elongation factor 1 alpha) and mitochondrial (cytochrome c oxidase subunit I) gene sequences of this and other major Phytophthora species. Congruence was observed in different phylogenetic data sets. Maximum likelihood phylogenetic reconstruction based on the different genes indicated that, although there is some variation between morphological and mating behaviour of P. inundata isolates, this taxon is a distinct and homogeneous species which always appeared as a sister taxon to P. humicola. Phytophthora inundata is shown to be a uniform species, with isolates from wide-ranging geographic origins having similar host ranges, molecular, and morphological characteristics.

    KW - intraspecific variation

    KW - Multiple gene genealogy

    KW - ECOSYSTEMS

    KW - TREES

    KW - SEQUENCES

    KW - MEGASPERMA

    KW - PATHOGEN

    KW - AUSTRALIA

    KW - NORTH-AMERICA

    KW - MULTIPLE

    KW - SP NOV.

    M3 - Article

    VL - 52

    SP - 46

    EP - 65

    JO - Phytopathologia mediterranea

    JF - Phytopathologia mediterranea

    SN - 0031-9465

    IS - 1

    ER -