Characterisation of selenium and tellurium nanoparticles produced by Aureobasidium pullulans using a multi-method approach

Kenneth C. Nwoko; Xinjin Liang; Magali Amj Perez; Eva Krupp; Geoffrey Michael Gadd; Jörg Feldmann

doi:10.1016/j.chroma.2021.462022

Characterisation of selenium and tellurium nanoparticles produced by Aureobasidium pullulans using a multi-method approach

Kenneth C. Nwoko, Xinjin Liang, Magali Amj Perez, Eva Krupp, Geoffrey Michael Gadd, Jörg Feldmann (Lead / Corresponding author)

Research output: Contribution to journal › Article › peer-review

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Abstract

Aureobasidium pullulans was grown in liquid culture media amended with selenite and tellurite and selenium (Se) and tellurium (Te) nanoparticles (NPs) were recovered after 30 d incubation. A separation method was applied to recover and characterise Se and Te NPs by asymmetric flow field flow fractionation (AF4) with online coupling to multi-angle light scattering (MALS), ultraviolet visible spectroscopy (UV-Vis), and inductively coupled plasma mass spectrometry (ICP-MS) detectors. Additional characterisation data was obtained from transmission electron microscopy (TEM), and dynamic light scattering (DLS). Solutions of 0.2% Novachem surfactant and 10 mM phosphate buffer were compared as mobile phases to investigate optimal AF4 separation and particle recovery using Se-NP as a model sample. 88% recovery was reported for 0.2% Novachem solution, compared with 50% recovery for phosphate buffer. Different crossflow (Cflow) rates were compared to further investigate optimum separation, with recoveries of 88% and 30% for Se-NPs, and 90% and 29% for Te-NPs for 3.5 mL min-1 and 2.5 mL min-1 respectively. Zeta-potential (ZP) data suggested higher stability for NP elution in Novachem solution, with increased stability attributed to minimised NP-membrane interaction due to PEGylation. Detection with MALS showed monodisperse Se-NPs (45-90 nm) and polydisperse Te-NPs (5-65 nm).Single particle ICP-MS showed mean particle diameters of 49.7 ± 2.7 nm, and 135 ± 4.3 nm, and limit of size detection (LOSD) of 20 nm and 45 nm for Se-NPs and Te-NPs respectively. TEM images of Se-NPs and Te-NPs displayed a spherical morphology, with the Te-NPs showing a clustered arrangement, which suggested electrostatic attraction amongst neighbouring particles. Particle hydrodynamic diameters (dH) measured with dynamic light scattering (DLS) further suggested monodisperse Se-NPs and polydisperse Te-NPs distributions, showing good agreement with AF4-MALS for Se-NPs, but suggests that the Rg obtained from AF4-MALS for Te-NP was unreliable. The results demonstrate a complementary application of asymmetric flow field-flow fractionation (AF4), ICP-MS, light scattering, UV-Vis detection, and microscopic techniques to characterise biogenic Se and Te NPs.

Original language	English
Article number	462022
Number of pages	10
Journal	Journal of Chromatography. A
Volume	1642
Early online date	27 Feb 2021
DOIs	https://doi.org/10.1016/j.chroma.2021.462022
Publication status	Published - 12 Apr 2021

Keywords

Biogenic nanoparticles
Selenium
Tellurium
AF4
ICP-MS/MS
spICP-MS

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/j.chroma.2021.462022Licence: Elsevier User Licence

Author Accepted ManuscriptAccepted author manuscript, 1.67 MBLicence: CC BY-NC-ND

Cite this

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title = "Characterisation of selenium and tellurium nanoparticles produced by Aureobasidium pullulans using a multi-method approach",

abstract = "Aureobasidium pullulans was grown in liquid culture media amended with selenite and tellurite and selenium (Se) and tellurium (Te) nanoparticles (NPs) were recovered after 30 d incubation. A separation method was applied to recover and characterise Se and Te NPs by asymmetric flow field flow fractionation (AF4) with online coupling to multi-angle light scattering (MALS), ultraviolet visible spectroscopy (UV-Vis), and inductively coupled plasma mass spectrometry (ICP-MS) detectors. Additional characterisation data was obtained from transmission electron microscopy (TEM), and dynamic light scattering (DLS). Solutions of 0.2% Novachem surfactant and 10 mM phosphate buffer were compared as mobile phases to investigate optimal AF4 separation and particle recovery using Se-NP as a model sample. 88% recovery was reported for 0.2% Novachem solution, compared with 50% recovery for phosphate buffer. Different crossflow (Cflow) rates were compared to further investigate optimum separation, with recoveries of 88% and 30% for Se-NPs, and 90% and 29% for Te-NPs for 3.5 mL min-1 and 2.5 mL min-1 respectively. Zeta-potential (ZP) data suggested higher stability for NP elution in Novachem solution, with increased stability attributed to minimised NP-membrane interaction due to PEGylation. Detection with MALS showed monodisperse Se-NPs (45-90 nm) and polydisperse Te-NPs (5-65 nm).Single particle ICP-MS showed mean particle diameters of 49.7 ± 2.7 nm, and 135 ± 4.3 nm, and limit of size detection (LOSD) of 20 nm and 45 nm for Se-NPs and Te-NPs respectively. TEM images of Se-NPs and Te-NPs displayed a spherical morphology, with the Te-NPs showing a clustered arrangement, which suggested electrostatic attraction amongst neighbouring particles. Particle hydrodynamic diameters (dH) measured with dynamic light scattering (DLS) further suggested monodisperse Se-NPs and polydisperse Te-NPs distributions, showing good agreement with AF4-MALS for Se-NPs, but suggests that the Rg obtained from AF4-MALS for Te-NP was unreliable. The results demonstrate a complementary application of asymmetric flow field-flow fractionation (AF4), ICP-MS, light scattering, UV-Vis detection, and microscopic techniques to characterise biogenic Se and Te NPs.",

keywords = "Biogenic nanoparticles, Selenium, Tellurium, AF4, ICP-MS/MS, spICP-MS",

author = "Nwoko, {Kenneth C.} and Xinjin Liang and Perez, {Magali Amj} and Eva Krupp and Gadd, {Geoffrey Michael} and J{\"o}rg Feldmann",

note = "Funding Information: KCN acknowledges the receipt of research funding received from the University of Aberdeen's Elphinstone PhD studentship award (RG13451), and the Niger-Delta Development Commission (NDDC) Postgraduate Foreign Scholarship (NDDC/DEHSS/2015PGFS/IMS/09). JF and KCN gratefully acknowledge Postnova Analytics , UK for loaning of the AF4-UV-MALS system and technical support; and the Microscopy and Histology Facility at the Institute of Medical Sciences (IMS) Foresterhill, University of Aberdeen for TEM measurements. G.M.G. gratefully acknowledges research support from Natural Environment Research Council ( NE/M010910/1 ) under the NERC Security of Supply of Mineral Resources Grant Programme: Tellurium and Selenium cycling and supply (TeaSe). Publisher Copyright: {\textcopyright} 2021 Elsevier B.V.",

year = "2021",

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day = "12",

doi = "10.1016/j.chroma.2021.462022",

language = "English",

volume = "1642",

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T1 - Characterisation of selenium and tellurium nanoparticles produced by Aureobasidium pullulans using a multi-method approach

AU - Nwoko, Kenneth C.

AU - Liang, Xinjin

AU - Perez, Magali Amj

AU - Krupp, Eva

AU - Gadd, Geoffrey Michael

AU - Feldmann, Jörg

N1 - Funding Information: KCN acknowledges the receipt of research funding received from the University of Aberdeen's Elphinstone PhD studentship award (RG13451), and the Niger-Delta Development Commission (NDDC) Postgraduate Foreign Scholarship (NDDC/DEHSS/2015PGFS/IMS/09). JF and KCN gratefully acknowledge Postnova Analytics , UK for loaning of the AF4-UV-MALS system and technical support; and the Microscopy and Histology Facility at the Institute of Medical Sciences (IMS) Foresterhill, University of Aberdeen for TEM measurements. G.M.G. gratefully acknowledges research support from Natural Environment Research Council ( NE/M010910/1 ) under the NERC Security of Supply of Mineral Resources Grant Programme: Tellurium and Selenium cycling and supply (TeaSe). Publisher Copyright: © 2021 Elsevier B.V.

PY - 2021/4/12

Y1 - 2021/4/12

N2 - Aureobasidium pullulans was grown in liquid culture media amended with selenite and tellurite and selenium (Se) and tellurium (Te) nanoparticles (NPs) were recovered after 30 d incubation. A separation method was applied to recover and characterise Se and Te NPs by asymmetric flow field flow fractionation (AF4) with online coupling to multi-angle light scattering (MALS), ultraviolet visible spectroscopy (UV-Vis), and inductively coupled plasma mass spectrometry (ICP-MS) detectors. Additional characterisation data was obtained from transmission electron microscopy (TEM), and dynamic light scattering (DLS). Solutions of 0.2% Novachem surfactant and 10 mM phosphate buffer were compared as mobile phases to investigate optimal AF4 separation and particle recovery using Se-NP as a model sample. 88% recovery was reported for 0.2% Novachem solution, compared with 50% recovery for phosphate buffer. Different crossflow (Cflow) rates were compared to further investigate optimum separation, with recoveries of 88% and 30% for Se-NPs, and 90% and 29% for Te-NPs for 3.5 mL min-1 and 2.5 mL min-1 respectively. Zeta-potential (ZP) data suggested higher stability for NP elution in Novachem solution, with increased stability attributed to minimised NP-membrane interaction due to PEGylation. Detection with MALS showed monodisperse Se-NPs (45-90 nm) and polydisperse Te-NPs (5-65 nm).Single particle ICP-MS showed mean particle diameters of 49.7 ± 2.7 nm, and 135 ± 4.3 nm, and limit of size detection (LOSD) of 20 nm and 45 nm for Se-NPs and Te-NPs respectively. TEM images of Se-NPs and Te-NPs displayed a spherical morphology, with the Te-NPs showing a clustered arrangement, which suggested electrostatic attraction amongst neighbouring particles. Particle hydrodynamic diameters (dH) measured with dynamic light scattering (DLS) further suggested monodisperse Se-NPs and polydisperse Te-NPs distributions, showing good agreement with AF4-MALS for Se-NPs, but suggests that the Rg obtained from AF4-MALS for Te-NP was unreliable. The results demonstrate a complementary application of asymmetric flow field-flow fractionation (AF4), ICP-MS, light scattering, UV-Vis detection, and microscopic techniques to characterise biogenic Se and Te NPs.

AB - Aureobasidium pullulans was grown in liquid culture media amended with selenite and tellurite and selenium (Se) and tellurium (Te) nanoparticles (NPs) were recovered after 30 d incubation. A separation method was applied to recover and characterise Se and Te NPs by asymmetric flow field flow fractionation (AF4) with online coupling to multi-angle light scattering (MALS), ultraviolet visible spectroscopy (UV-Vis), and inductively coupled plasma mass spectrometry (ICP-MS) detectors. Additional characterisation data was obtained from transmission electron microscopy (TEM), and dynamic light scattering (DLS). Solutions of 0.2% Novachem surfactant and 10 mM phosphate buffer were compared as mobile phases to investigate optimal AF4 separation and particle recovery using Se-NP as a model sample. 88% recovery was reported for 0.2% Novachem solution, compared with 50% recovery for phosphate buffer. Different crossflow (Cflow) rates were compared to further investigate optimum separation, with recoveries of 88% and 30% for Se-NPs, and 90% and 29% for Te-NPs for 3.5 mL min-1 and 2.5 mL min-1 respectively. Zeta-potential (ZP) data suggested higher stability for NP elution in Novachem solution, with increased stability attributed to minimised NP-membrane interaction due to PEGylation. Detection with MALS showed monodisperse Se-NPs (45-90 nm) and polydisperse Te-NPs (5-65 nm).Single particle ICP-MS showed mean particle diameters of 49.7 ± 2.7 nm, and 135 ± 4.3 nm, and limit of size detection (LOSD) of 20 nm and 45 nm for Se-NPs and Te-NPs respectively. TEM images of Se-NPs and Te-NPs displayed a spherical morphology, with the Te-NPs showing a clustered arrangement, which suggested electrostatic attraction amongst neighbouring particles. Particle hydrodynamic diameters (dH) measured with dynamic light scattering (DLS) further suggested monodisperse Se-NPs and polydisperse Te-NPs distributions, showing good agreement with AF4-MALS for Se-NPs, but suggests that the Rg obtained from AF4-MALS for Te-NP was unreliable. The results demonstrate a complementary application of asymmetric flow field-flow fractionation (AF4), ICP-MS, light scattering, UV-Vis detection, and microscopic techniques to characterise biogenic Se and Te NPs.

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KW - Selenium

KW - Tellurium

KW - AF4

KW - ICP-MS/MS

KW - spICP-MS

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ER -

Characterisation of selenium and tellurium nanoparticles produced by Aureobasidium pullulans using a multi-method approach

Abstract

Keywords

ASJC Scopus subject areas

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Tellurium and Selenium Cycling and Supply (Joint with Universities of Leicester, Durham, Edinburgh, Cardiff, Aberdeen and Open University and Natural History Museum)

COG3: The Geology, Geometallurgy and Geomicrobiology of Cobalt Resources Leading to New Product Streams (joint with Natural History Museum and Universities of Manchester, Bangor, Exeter, Loughborough and Southampton and Industrial Partner)

Cite this

Characterisation of selenium and tellurium nanoparticles produced by Aureobasidium pullulans using a multi-method approach

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Projects

Tellurium and Selenium Cycling and Supply (Joint with Universities of Leicester, Durham, Edinburgh, Cardiff, Aberdeen and Open University and Natural History Museum)

COG3: The Geology, Geometallurgy and Geomicrobiology of Cobalt Resources Leading to New Product Streams (joint with Natural History Museum and Universities of Manchester, Bangor, Exeter, Loughborough and Southampton and Industrial Partner)

Cite this