Characterisation of the Escherichia coli mfd promoter

Louise K. Stanley, Nigel J. Savery (Lead / Corresponding author)

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)


    The bacterial mfd gene encodes a transcription-repair coupling factor that mediates the preferential repair of DNA damage in the template strand of active transcriptional units. In this report, the transcription start site for the Escherichia coli mfd gene was determined in vivo and in vitro, and the DNA determinants for mfd transcription by deletion and site-directed mutagenesis were defined. A canonical σ70-dependent promoter, mfd P1, was responsible for the majority of mfd transcription, and a core region consisting of residues -42 to +5 was sufficient for full activity in rich and minimal media.

    Original languageEnglish
    Pages (from-to)381-385
    Number of pages5
    JournalArchives of Microbiology
    Issue number5
    Early online date26 Mar 2003
    Publication statusPublished - May 2003


    • Escherichia coli
    • Mfd
    • Promoter
    • Transcription initiation
    • Transcription-coupled repair
    • TRCF

    ASJC Scopus subject areas

    • Microbiology
    • Biochemistry
    • Molecular Biology
    • Genetics


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