Characterisation of the Escherichia coli mfd promoter

Louise K. Stanley; Nigel J. Savery

doi:10.1007/s00203-003-0540-9

Characterisation of the Escherichia coli mfd promoter

Louise K. Stanley, Nigel J. Savery (Lead / Corresponding author)

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The bacterial mfd gene encodes a transcription-repair coupling factor that mediates the preferential repair of DNA damage in the template strand of active transcriptional units. In this report, the transcription start site for the Escherichia coli mfd gene was determined in vivo and in vitro, and the DNA determinants for mfd transcription by deletion and site-directed mutagenesis were defined. A canonical σ⁷⁰-dependent promoter, mfd P1, was responsible for the majority of mfd transcription, and a core region consisting of residues -42 to +5 was sufficient for full activity in rich and minimal media.

Original language	English
Pages (from-to)	381-385
Number of pages	5
Journal	Archives of Microbiology
Volume	179
Issue number	5
Early online date	26 Mar 2003
DOIs	https://doi.org/10.1007/s00203-003-0540-9
Publication status	Published - May 2003

Keywords

Escherichia coli
Mfd
Promoter
Transcription initiation
Transcription-coupled repair
TRCF

ASJC Scopus subject areas

Microbiology
Biochemistry
Molecular Biology
Genetics

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10.1007/s00203-003-0540-9

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abstract = "The bacterial mfd gene encodes a transcription-repair coupling factor that mediates the preferential repair of DNA damage in the template strand of active transcriptional units. In this report, the transcription start site for the Escherichia coli mfd gene was determined in vivo and in vitro, and the DNA determinants for mfd transcription by deletion and site-directed mutagenesis were defined. A canonical σ70-dependent promoter, mfd P1, was responsible for the majority of mfd transcription, and a core region consisting of residues -42 to +5 was sufficient for full activity in rich and minimal media.",

keywords = "Escherichia coli, Mfd, Promoter, Transcription initiation, Transcription-coupled repair, TRCF",

author = "Stanley, {Louise K.} and Savery, {Nigel J.}",

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AU - Savery, Nigel J.

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N2 - The bacterial mfd gene encodes a transcription-repair coupling factor that mediates the preferential repair of DNA damage in the template strand of active transcriptional units. In this report, the transcription start site for the Escherichia coli mfd gene was determined in vivo and in vitro, and the DNA determinants for mfd transcription by deletion and site-directed mutagenesis were defined. A canonical σ70-dependent promoter, mfd P1, was responsible for the majority of mfd transcription, and a core region consisting of residues -42 to +5 was sufficient for full activity in rich and minimal media.

AB - The bacterial mfd gene encodes a transcription-repair coupling factor that mediates the preferential repair of DNA damage in the template strand of active transcriptional units. In this report, the transcription start site for the Escherichia coli mfd gene was determined in vivo and in vitro, and the DNA determinants for mfd transcription by deletion and site-directed mutagenesis were defined. A canonical σ70-dependent promoter, mfd P1, was responsible for the majority of mfd transcription, and a core region consisting of residues -42 to +5 was sufficient for full activity in rich and minimal media.

KW - Escherichia coli

KW - Mfd

KW - Promoter

KW - Transcription initiation

KW - Transcription-coupled repair

KW - TRCF

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Characterisation of the Escherichia coli mfd promoter

Abstract

Keywords

ASJC Scopus subject areas

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