Characterisation of the mob locus from Rhodobacter sphaeroides required for molybdenum cofactor biosynthesis

Tracy Palmer, Ian P G Goodfellow, R. Elizabeth Sockett, Alastair G McEwan, David H Boxer

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    15 Citations (Scopus)

    Abstract

    A clone carrying the mob locus from Rb. sphaeroides WS8 has been isolated from a cosmid library by Southern blotting with a probe covering the mob genes of Escherichia coli. The mob DNA has been subcloned and partially restores molybdoenzyme activities when transformed into E. coli mob strains. DNA sequence analysis of the subclone carrying the mob genes predicted at least 2 open reading frames. The mobA gene encodes protein FA whilst mobB encodes a nucleotide binding protein which has at least one extra domain relative to its E. coli counterpart. (C) 1998 Elsevier Science B.V.

    Original languageEnglish
    Pages (from-to)135-140
    Number of pages6
    JournalBiochimica et Biophysica Acta-Gene Structure and Expression
    Volume1395
    Issue number2
    DOIs
    Publication statusPublished - 21 Jan 1998

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