Characterisation of the mob locus from Rhodobacter sphaeroides required for molybdenum cofactor biosynthesis

Tracy Palmer; Ian P G  Goodfellow; R. Elizabeth  Sockett; Alastair G  McEwan; David H  Boxer

doi:10.1016/S0167-4781(97)00145-0

Characterisation of the mob locus from Rhodobacter sphaeroides required for molybdenum cofactor biosynthesis

Tracy Palmer, Ian P G Goodfellow, R. Elizabeth Sockett, Alastair G McEwan, David H Boxer

Research output: Contribution to journal › Article › peer-review

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Abstract

A clone carrying the mob locus from Rb. sphaeroides WS8 has been isolated from a cosmid library by Southern blotting with a probe covering the mob genes of Escherichia coli. The mob DNA has been subcloned and partially restores molybdoenzyme activities when transformed into E. coli mob strains. DNA sequence analysis of the subclone carrying the mob genes predicted at least 2 open reading frames. The mobA gene encodes protein FA whilst mobB encodes a nucleotide binding protein which has at least one extra domain relative to its E. coli counterpart. (C) 1998 Elsevier Science B.V.

Original language	English
Pages (from-to)	135-140
Number of pages	6
Journal	Biochimica et Biophysica Acta-Gene Structure and Expression
Volume	1395
Issue number	2
DOIs	https://doi.org/10.1016/S0167-4781(97)00145-0
Publication status	Published - 21 Jan 1998

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title = "Characterisation of the mob locus from Rhodobacter sphaeroides required for molybdenum cofactor biosynthesis",

abstract = "A clone carrying the mob locus from Rb. sphaeroides WS8 has been isolated from a cosmid library by Southern blotting with a probe covering the mob genes of Escherichia coli. The mob DNA has been subcloned and partially restores molybdoenzyme activities when transformed into E. coli mob strains. DNA sequence analysis of the subclone carrying the mob genes predicted at least 2 open reading frames. The mobA gene encodes protein FA whilst mobB encodes a nucleotide binding protein which has at least one extra domain relative to its E. coli counterpart. (C) 1998 Elsevier Science B.V.",

author = "Tracy Palmer and Goodfellow, {Ian P G} and Sockett, {R. Elizabeth} and McEwan, {Alastair G} and Boxer, {David H}",

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T1 - Characterisation of the mob locus from Rhodobacter sphaeroides required for molybdenum cofactor biosynthesis

AU - Palmer, Tracy

AU - Goodfellow, Ian P G

AU - Sockett, R. Elizabeth

AU - McEwan, Alastair G

AU - Boxer, David H

PY - 1998/1/21

Y1 - 1998/1/21

N2 - A clone carrying the mob locus from Rb. sphaeroides WS8 has been isolated from a cosmid library by Southern blotting with a probe covering the mob genes of Escherichia coli. The mob DNA has been subcloned and partially restores molybdoenzyme activities when transformed into E. coli mob strains. DNA sequence analysis of the subclone carrying the mob genes predicted at least 2 open reading frames. The mobA gene encodes protein FA whilst mobB encodes a nucleotide binding protein which has at least one extra domain relative to its E. coli counterpart. (C) 1998 Elsevier Science B.V.

AB - A clone carrying the mob locus from Rb. sphaeroides WS8 has been isolated from a cosmid library by Southern blotting with a probe covering the mob genes of Escherichia coli. The mob DNA has been subcloned and partially restores molybdoenzyme activities when transformed into E. coli mob strains. DNA sequence analysis of the subclone carrying the mob genes predicted at least 2 open reading frames. The mobA gene encodes protein FA whilst mobB encodes a nucleotide binding protein which has at least one extra domain relative to its E. coli counterpart. (C) 1998 Elsevier Science B.V.

U2 - 10.1016/S0167-4781(97)00145-0

DO - 10.1016/S0167-4781(97)00145-0

M3 - Article

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SP - 135

EP - 140

JO - Biochimica et Biophysica Acta-Gene Structure and Expression

JF - Biochimica et Biophysica Acta-Gene Structure and Expression

IS - 2

ER -

Characterisation of the mob locus from Rhodobacter sphaeroides required for molybdenum cofactor biosynthesis

Abstract

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