Characterization and validation of an isotope-dilution LC-MS/MS method for quantification of total desmosine and isodesmosine in plasma and serum

Osama Albarbarawi, Alun Barton, Douglas Miller, Charles McSharry, Rekha Chaudhuri, Neil C. Thomson, Colin N. A. Palmer, Graham Devereux, Jeffrey T-J Huang (Lead / Corresponding author)

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background: Desmosine/isodesmosine (DES/IDS) is a promising biomarker for estimating activity of elastin degradation. Results/methodology: A stable isotope dilution LC-MS/MS method for measuring serum/plasma DES/IDS was developed and validated. The reportable range of this assay was 0.1-160 ng/ml. Serum/plasma DES/IDS level was stable at room temperature or 4°C for 20 h, and for three freeze-thaw cycles. Interferences from endogenous compounds and ion suppression/enhancing effect were also evaluated. Our results suggest the absolute necessity of using an IS in the measurement. We found that serum/plasma DES/IDS levels from patients with chronic obstructive pulmonary disease and cystic fibrosis were significantly higher compared with healthy smokers. Conclusion: These results demonstrate that the LC-MS/MS method provides sensitive, reproducible and accurate quantification of total serum/plasma DES/IDS.
Original languageEnglish
Pages (from-to)1991-2001
Number of pages11
JournalBioanalysis
Volume5
Issue number16
DOIs
Publication statusPublished - Aug 2013

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