Characterization and validation of an isotope-dilution LC-MS/MS method for quantification of total desmosine and isodesmosine in plasma and serum

Osama Albarbarawi; Alun Barton; Douglas Miller; Charles McSharry; Rekha Chaudhuri; Neil C. Thomson; Colin N. A. Palmer; Graham Devereux; Jeffrey T-J Huang

doi:10.4155/bio.13.164

Characterization and validation of an isotope-dilution LC-MS/MS method for quantification of total desmosine and isodesmosine in plasma and serum

Osama Albarbarawi, Alun Barton, Douglas Miller, Charles McSharry, Rekha Chaudhuri, Neil C. Thomson, Colin N. A. Palmer, Graham Devereux, Jeffrey T-J Huang (Lead / Corresponding author)

Biomarker and Drug Analysis Laboratory

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

Background: Desmosine/isodesmosine (DES/IDS) is a promising biomarker for estimating activity of elastin degradation. Results/methodology: A stable isotope dilution LC-MS/MS method for measuring serum/plasma DES/IDS was developed and validated. The reportable range of this assay was 0.1-160 ng/ml. Serum/plasma DES/IDS level was stable at room temperature or 4°C for 20 h, and for three freeze-thaw cycles. Interferences from endogenous compounds and ion suppression/enhancing effect were also evaluated. Our results suggest the absolute necessity of using an IS in the measurement. We found that serum/plasma DES/IDS levels from patients with chronic obstructive pulmonary disease and cystic fibrosis were significantly higher compared with healthy smokers. Conclusion: These results demonstrate that the LC-MS/MS method provides sensitive, reproducible and accurate quantification of total serum/plasma DES/IDS.

Original language	English
Pages (from-to)	1991-2001
Number of pages	11
Journal	Bioanalysis
Volume	5
Issue number	16
DOIs	https://doi.org/10.4155/bio.13.164
Publication status	Published - Aug 2013

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10.4155/bio.13.164

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title = "Characterization and validation of an isotope-dilution LC-MS/MS method for quantification of total desmosine and isodesmosine in plasma and serum",

abstract = "Background: Desmosine/isodesmosine (DES/IDS) is a promising biomarker for estimating activity of elastin degradation. Results/methodology: A stable isotope dilution LC-MS/MS method for measuring serum/plasma DES/IDS was developed and validated. The reportable range of this assay was 0.1-160 ng/ml. Serum/plasma DES/IDS level was stable at room temperature or 4°C for 20 h, and for three freeze-thaw cycles. Interferences from endogenous compounds and ion suppression/enhancing effect were also evaluated. Our results suggest the absolute necessity of using an IS in the measurement. We found that serum/plasma DES/IDS levels from patients with chronic obstructive pulmonary disease and cystic fibrosis were significantly higher compared with healthy smokers. Conclusion: These results demonstrate that the LC-MS/MS method provides sensitive, reproducible and accurate quantification of total serum/plasma DES/IDS.",

author = "Osama Albarbarawi and Alun Barton and Douglas Miller and Charles McSharry and Rekha Chaudhuri and Thomson, {Neil C.} and Palmer, {Colin N. A.} and Graham Devereux and Huang, {Jeffrey T-J}",

year = "2013",

month = aug,

doi = "10.4155/bio.13.164",

language = "English",

volume = "5",

pages = "1991--2001",

journal = "Bioanalysis",

issn = "1757-6199",

publisher = "Taylor & Francis",

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TY - JOUR

T1 - Characterization and validation of an isotope-dilution LC-MS/MS method for quantification of total desmosine and isodesmosine in plasma and serum

AU - Albarbarawi, Osama

AU - Barton, Alun

AU - Miller, Douglas

AU - McSharry, Charles

AU - Chaudhuri, Rekha

AU - Thomson, Neil C.

AU - Palmer, Colin N. A.

AU - Devereux, Graham

AU - Huang, Jeffrey T-J

PY - 2013/8

Y1 - 2013/8

N2 - Background: Desmosine/isodesmosine (DES/IDS) is a promising biomarker for estimating activity of elastin degradation. Results/methodology: A stable isotope dilution LC-MS/MS method for measuring serum/plasma DES/IDS was developed and validated. The reportable range of this assay was 0.1-160 ng/ml. Serum/plasma DES/IDS level was stable at room temperature or 4°C for 20 h, and for three freeze-thaw cycles. Interferences from endogenous compounds and ion suppression/enhancing effect were also evaluated. Our results suggest the absolute necessity of using an IS in the measurement. We found that serum/plasma DES/IDS levels from patients with chronic obstructive pulmonary disease and cystic fibrosis were significantly higher compared with healthy smokers. Conclusion: These results demonstrate that the LC-MS/MS method provides sensitive, reproducible and accurate quantification of total serum/plasma DES/IDS.

AB - Background: Desmosine/isodesmosine (DES/IDS) is a promising biomarker for estimating activity of elastin degradation. Results/methodology: A stable isotope dilution LC-MS/MS method for measuring serum/plasma DES/IDS was developed and validated. The reportable range of this assay was 0.1-160 ng/ml. Serum/plasma DES/IDS level was stable at room temperature or 4°C for 20 h, and for three freeze-thaw cycles. Interferences from endogenous compounds and ion suppression/enhancing effect were also evaluated. Our results suggest the absolute necessity of using an IS in the measurement. We found that serum/plasma DES/IDS levels from patients with chronic obstructive pulmonary disease and cystic fibrosis were significantly higher compared with healthy smokers. Conclusion: These results demonstrate that the LC-MS/MS method provides sensitive, reproducible and accurate quantification of total serum/plasma DES/IDS.

U2 - 10.4155/bio.13.164

DO - 10.4155/bio.13.164

M3 - Article

C2 - 23937134

SN - 1757-6199

VL - 5

SP - 1991

EP - 2001

JO - Bioanalysis

JF - Bioanalysis

IS - 16

ER -

Characterization and validation of an isotope-dilution LC-MS/MS method for quantification of total desmosine and isodesmosine in plasma and serum

Abstract

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