Characterization of a cDNA encoding a human kidney, cytochrome P-450 4A fatty acid ω-hydroxylase and the cognate enzyme expressed in Escherichia coli

Colin N.A. Palmer; Toby H. Richardson; Keith J. Griffin; Mei Hui Hsu; A. Scott Muerhoff; Joan E. Clark; Eric F. Johnson

doi:10.1016/0167-4781(93)90285-L

Characterization of a cDNA encoding a human kidney, cytochrome P-450 4A fatty acid ω-hydroxylase and the cognate enzyme expressed in Escherichia coli

Colin N.A. Palmer, Toby H. Richardson, Keith J. Griffin, Mei Hui Hsu, A. Scott Muerhoff, Joan E. Clark, Eric F. Johnson (Lead / Corresponding author)

Population Health and Genomics

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86 Citations (Scopus)

Abstract

A cDNA encoding a cytochrome P-450 4A (CYP4AII) was cloned from a human kidney cDNA library. Northern blot analysis and RNase protection assays indicate that related mRNAs occur in kidney and liver with the highest abundance found in kidney. The enzyme was expressed from its cDNA in Escherichia coli. A solubilized preparation of the enzyme reconstituted with cytochrome P-450 reductase catalyzed the ω-hydroxylation of lauric acid, palmitic acid, and arachidonic acid with turnover numbers of 9.8, 2.2 and 0.55 min^-1, respectively. Little or no activity was detected toward prostaglandins A₁ and E₁.

Original language	English
Pages (from-to)	161-166
Number of pages	6
Journal	BBA - Gene Structure and Expression
Volume	1172
Issue number	1-2
DOIs	https://doi.org/10.1016/0167-4781(93)90285-L
Publication status	Published - 20 Feb 1993

Keywords

(Human kidney)
Arachidonic acid
CYP4AII
Cytochrome P-450
Fatty acid
ω-Hydroxylase

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/0167-4781(93)90285-L

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Palmer, C. N. A., Richardson, T. H., Griffin, K. J., Hsu, M. H., Muerhoff, A. S., Clark, J. E., & Johnson, E. F. (1993). Characterization of a cDNA encoding a human kidney, cytochrome P-450 4A fatty acid ω-hydroxylase and the cognate enzyme expressed in Escherichia coli. BBA - Gene Structure and Expression, 1172(1-2), 161-166. https://doi.org/10.1016/0167-4781(93)90285-L

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abstract = "A cDNA encoding a cytochrome P-450 4A (CYP4AII) was cloned from a human kidney cDNA library. Northern blot analysis and RNase protection assays indicate that related mRNAs occur in kidney and liver with the highest abundance found in kidney. The enzyme was expressed from its cDNA in Escherichia coli. A solubilized preparation of the enzyme reconstituted with cytochrome P-450 reductase catalyzed the ω-hydroxylation of lauric acid, palmitic acid, and arachidonic acid with turnover numbers of 9.8, 2.2 and 0.55 min-1, respectively. Little or no activity was detected toward prostaglandins A1 and E1.",

keywords = "(Human kidney), Arachidonic acid, CYP4AII, Cytochrome P-450, Fatty acid, ω-Hydroxylase",

author = "Palmer, {Colin N.A.} and Richardson, {Toby H.} and Griffin, {Keith J.} and Hsu, {Mei Hui} and Muerhoff, {A. Scott} and Clark, {Joan E.} and Johnson, {Eric F.}",

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Characterization of a cDNA encoding a human kidney, cytochrome P-450 4A fatty acid ω-hydroxylase and the cognate enzyme expressed in Escherichia coli. / Palmer, Colin N.A.; Richardson, Toby H.; Griffin, Keith J. et al.
In: BBA - Gene Structure and Expression, Vol. 1172, No. 1-2, 20.02.1993, p. 161-166.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Characterization of a cDNA encoding a human kidney, cytochrome P-450 4A fatty acid ω-hydroxylase and the cognate enzyme expressed in Escherichia coli

AU - Palmer, Colin N.A.

AU - Richardson, Toby H.

AU - Griffin, Keith J.

AU - Hsu, Mei Hui

AU - Muerhoff, A. Scott

AU - Clark, Joan E.

AU - Johnson, Eric F.

PY - 1993/2/20

Y1 - 1993/2/20

N2 - A cDNA encoding a cytochrome P-450 4A (CYP4AII) was cloned from a human kidney cDNA library. Northern blot analysis and RNase protection assays indicate that related mRNAs occur in kidney and liver with the highest abundance found in kidney. The enzyme was expressed from its cDNA in Escherichia coli. A solubilized preparation of the enzyme reconstituted with cytochrome P-450 reductase catalyzed the ω-hydroxylation of lauric acid, palmitic acid, and arachidonic acid with turnover numbers of 9.8, 2.2 and 0.55 min-1, respectively. Little or no activity was detected toward prostaglandins A1 and E1.

AB - A cDNA encoding a cytochrome P-450 4A (CYP4AII) was cloned from a human kidney cDNA library. Northern blot analysis and RNase protection assays indicate that related mRNAs occur in kidney and liver with the highest abundance found in kidney. The enzyme was expressed from its cDNA in Escherichia coli. A solubilized preparation of the enzyme reconstituted with cytochrome P-450 reductase catalyzed the ω-hydroxylation of lauric acid, palmitic acid, and arachidonic acid with turnover numbers of 9.8, 2.2 and 0.55 min-1, respectively. Little or no activity was detected toward prostaglandins A1 and E1.

KW - (Human kidney)

KW - Arachidonic acid

KW - CYP4AII

KW - Cytochrome P-450

KW - Fatty acid

KW - ω-Hydroxylase

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DO - 10.1016/0167-4781(93)90285-L

M3 - Article

C2 - 7679927

AN - SCOPUS:0027459403

SN - 0167-4781

VL - 1172

SP - 161

EP - 166

JO - BBA - Gene Structure and Expression

JF - BBA - Gene Structure and Expression

IS - 1-2

ER -

Characterization of a cDNA encoding a human kidney, cytochrome P-450 4A fatty acid ω-hydroxylase and the cognate enzyme expressed in Escherichia coli

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ASJC Scopus subject areas

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