Characterization of a cDNA encoding a human kidney, cytochrome P-450 4A fatty acid ω-hydroxylase and the cognate enzyme expressed in Escherichia coli

Colin N.A. Palmer, Toby H. Richardson, Keith J. Griffin, Mei Hui Hsu, A. Scott Muerhoff, Joan E. Clark, Eric F. Johnson (Lead / Corresponding author)

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

A cDNA encoding a cytochrome P-450 4A (CYP4AII) was cloned from a human kidney cDNA library. Northern blot analysis and RNase protection assays indicate that related mRNAs occur in kidney and liver with the highest abundance found in kidney. The enzyme was expressed from its cDNA in Escherichia coli. A solubilized preparation of the enzyme reconstituted with cytochrome P-450 reductase catalyzed the ω-hydroxylation of lauric acid, palmitic acid, and arachidonic acid with turnover numbers of 9.8, 2.2 and 0.55 min-1, respectively. Little or no activity was detected toward prostaglandins A1 and E1.

Original languageEnglish
Pages (from-to)161-166
Number of pages6
JournalBBA - Gene Structure and Expression
Volume1172
Issue number1-2
DOIs
Publication statusPublished - 20 Feb 1993

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Cytochrome P-450 CYP4A
lauric acid
Mixed Function Oxygenases
Escherichia coli
Fatty Acids
Complementary DNA
Kidney
NADPH-Ferrihemoprotein Reductase
Hydroxylation
Palmitic Acid
Alprostadil
Enzymes
Ribonucleases
Gene Library
Arachidonic Acid
Liver
Assays
Northern Blotting
Messenger RNA

Keywords

  • (Human kidney)
  • Arachidonic acid
  • CYP4AII
  • Cytochrome P-450
  • Fatty acid
  • ω-Hydroxylase

Cite this

Palmer, Colin N.A. ; Richardson, Toby H. ; Griffin, Keith J. ; Hsu, Mei Hui ; Muerhoff, A. Scott ; Clark, Joan E. ; Johnson, Eric F. / Characterization of a cDNA encoding a human kidney, cytochrome P-450 4A fatty acid ω-hydroxylase and the cognate enzyme expressed in Escherichia coli. In: BBA - Gene Structure and Expression. 1993 ; Vol. 1172, No. 1-2. pp. 161-166.
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Characterization of a cDNA encoding a human kidney, cytochrome P-450 4A fatty acid ω-hydroxylase and the cognate enzyme expressed in Escherichia coli. / Palmer, Colin N.A.; Richardson, Toby H.; Griffin, Keith J.; Hsu, Mei Hui; Muerhoff, A. Scott; Clark, Joan E.; Johnson, Eric F. (Lead / Corresponding author).

In: BBA - Gene Structure and Expression, Vol. 1172, No. 1-2, 20.02.1993, p. 161-166.

Research output: Contribution to journalArticle

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AU - Palmer, Colin N.A.

AU - Richardson, Toby H.

AU - Griffin, Keith J.

AU - Hsu, Mei Hui

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AU - Johnson, Eric F.

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KW - Arachidonic acid

KW - CYP4AII

KW - Cytochrome P-450

KW - Fatty acid

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