Characterization of a new SUMO-1 nuclear body (SNB) enriched in pCREB, CBP, c-Jun in neuron-like UR61 cells

Joaquin Navascues, Rocio Bengoechea, Olga Tapia, José P Vaqué, Miguel Lafarga, Maria T Berciano

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    The neuron-like UR61 cell is a stable PC12 subline that contains a mouse N-ras oncogene. Dexamethasone (Dex) treatment induces a neuron-like differentiation, which is associated with neuritogenesis and nuclear expression of the glucocorticoid receptor and c-Jun. In differentiated UR61 cells, small ubiquitin-like modifiers 1 (SUMO-1) is concentrated in a new category of SUMO-1 nuclear bodies (SNBs) distinct from promyelocytic leukemia (PML) bodies by their large size and absence of PML protein. SNBs are 1 to 3 mum in diameter and exhibit a fine granular texture by electron microscopy. They are free of splicing factors and transcription foci and show spatial associations with Cajal bodies. In addition to SUMO-1 and the E2-conjugating enzyme Ubc9, which is essential for sumoylation, SNBs concentrate the transcriptional regulators CBP, CREB, and c-Jun. Moreover, transfection experiments demonstrate that SNBs accumulate the active conjugating form of SUMO-1 but not the conjugation defective variant of SUMO-1, supporting that SNBs are sites of sumoylation. Our results suggest that SNBs play a role in the control of the nucleoplasmic concentration of transcription regulators involved in neuroprotection and survival of the UR61 cells.
    Original languageEnglish
    Pages (from-to)441-451
    Number of pages11
    JournalChromosoma
    Volume116
    Issue number5
    DOIs
    Publication statusPublished - 2007

    Keywords

    • Animals
    • CREB-Binding Protein
    • Cell Nucleolus
    • Cells, Cultured
    • Dexamethasone
    • Gene Expression Regulation
    • JNK Mitogen-Activated Protein Kinases
    • Neurons
    • PC12 Cells
    • Rats
    • SUMO-1 Protein

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  • Cite this

    Navascues, J., Bengoechea, R., Tapia, O., Vaqué, J. P., Lafarga, M., & Berciano, M. T. (2007). Characterization of a new SUMO-1 nuclear body (SNB) enriched in pCREB, CBP, c-Jun in neuron-like UR61 cells. Chromosoma, 116(5), 441-451. https://doi.org/10.1007/s00412-007-0107-7