Characterization of a novel phosphatidylinositol 3-phosphate-binding protein containing two FYVE fingers in tandem that is targeted to the Golgi

P. C.F. Cheung (Lead / Corresponding author), L. Trinkle-Mulcahy, P. Cohen, J. M. Lucocq

    Research output: Contribution to journalArticle

    23 Citations (Scopus)

    Abstract

    We have identified a novel protein of predicted-molecular mass 40 kDa that contains two FYVE domains in tandem and has therefore been named TAFFI (TAndem FYVE Fingers:1). The protein is expressed predominantly in heart and binds to PtdIns3P specifically, even though the FYVE domains in TAFF1 lacks the first Arg of the consensus sequence R(K/R)HHCR, critical for the PtdIns3P binding of other FYVE domains identified so far. The first Arg is replaced by a Thr and Ser in the N-terminal and C-terminal FYVE domains of TAFF1 respectively. Mutational analysis indicates that both FYVE domains are required for high affinity binding to PtdIns3P. Cell localization studies using a green fluorescent protein fusion show that TAFF1 is localized to the Golgi, and that the Golgi targeting sequence is located within the N-terminal 187 residues and not in either FYYE domain.

    Original languageEnglish
    Pages (from-to)113-121
    Number of pages9
    JournalBiochemical Journal
    Volume355
    Issue number1
    DOIs
    Publication statusPublished - 1 Apr 2001

    Keywords

    • DFCP1
    • EEA1
    • Inositol phospholipid
    • TAFF1

    Fingerprint Dive into the research topics of 'Characterization of a novel phosphatidylinositol 3-phosphate-binding protein containing two FYVE fingers in tandem that is targeted to the Golgi'. Together they form a unique fingerprint.

  • Cite this