Characterization of a novel phosphatidylinositol 3-phosphate-binding protein containing two FYVE fingers in tandem that is targeted to the Golgi

TY - JOUR

T1 - Characterization of a novel phosphatidylinositol 3-phosphate-binding protein containing two FYVE fingers in tandem that is targeted to the Golgi

AU - Cheung, P. C.F.

AU - Trinkle-Mulcahy, L.

AU - Cohen, P.

AU - Lucocq, J. M.

PY - 2001/4/1

Y1 - 2001/4/1

N2 - We have identified a novel protein of predicted-molecular mass 40 kDa that contains two FYVE domains in tandem and has therefore been named TAFFI (TAndem FYVE Fingers:1). The protein is expressed predominantly in heart and binds to PtdIns3P specifically, even though the FYVE domains in TAFF1 lacks the first Arg of the consensus sequence R(K/R)HHCR, critical for the PtdIns3P binding of other FYVE domains identified so far. The first Arg is replaced by a Thr and Ser in the N-terminal and C-terminal FYVE domains of TAFF1 respectively. Mutational analysis indicates that both FYVE domains are required for high affinity binding to PtdIns3P. Cell localization studies using a green fluorescent protein fusion show that TAFF1 is localized to the Golgi, and that the Golgi targeting sequence is located within the N-terminal 187 residues and not in either FYYE domain.

AB - We have identified a novel protein of predicted-molecular mass 40 kDa that contains two FYVE domains in tandem and has therefore been named TAFFI (TAndem FYVE Fingers:1). The protein is expressed predominantly in heart and binds to PtdIns3P specifically, even though the FYVE domains in TAFF1 lacks the first Arg of the consensus sequence R(K/R)HHCR, critical for the PtdIns3P binding of other FYVE domains identified so far. The first Arg is replaced by a Thr and Ser in the N-terminal and C-terminal FYVE domains of TAFF1 respectively. Mutational analysis indicates that both FYVE domains are required for high affinity binding to PtdIns3P. Cell localization studies using a green fluorescent protein fusion show that TAFF1 is localized to the Golgi, and that the Golgi targeting sequence is located within the N-terminal 187 residues and not in either FYYE domain.

KW - DFCP1

KW - EEA1

KW - Inositol phospholipid

KW - TAFF1

UR - https://www.scopus.com/pages/publications/0035310983

U2 - 10.1042/0264-6021:3550113

DO - 10.1042/0264-6021:3550113

M3 - Article

C2 - 11256955

AN - SCOPUS:0035310983

SN - 0264-6021

VL - 355

SP - 113

EP - 121

JO - Biochemical Journal

JF - Biochemical Journal

IS - 1

ER -