Characterization of Phytophthora infestans populations in northwestern Algeria during 2008-2014

Fatma Zohra Rekad; David Edward Llewelyn Cooke; Ivana Puglisi; Eva Randall; Yamina Guenaoui; Zouaoui Bouznad; Maria Evoli; Antonella Pane; Leonardo Schena; Gaetano Magnano di San Lio; Santa Olga Cacciola

doi:10.1016/j.funbio.2017.01.004

Characterization of Phytophthora infestans populations in northwestern Algeria during 2008-2014

Fatma Zohra Rekad (Lead / Corresponding author), David Edward Llewelyn Cooke, Ivana Puglisi, Eva Randall, Yamina Guenaoui, Zouaoui Bouznad, Maria Evoli, Antonella Pane, Leonardo Schena, Gaetano Magnano di San Lio, Santa Olga Cacciola (Lead / Corresponding author)

Plant Sciences

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

A total of 161 Phytophthora infestans isolates, collected from infected potato and tomato plants during 2008-2014, were characterized based on mating type, metalaxyl sensitivity and polymorphism at 12 simple sequence repeat (SSR) loci, in order to investigate the population of P. infestans in the north-west of Algeria, an emerging potato production region. The majority of isolates were of A2 mating type (112 isolates). A high percentage (89 %) of resistance to metalaxyl among isolates was detected. The metalaxyl resistant phenotype was present in both mating types with a higher percentage in A2 mating type isolates. SSR-based genotypic analysis of P. infestans population showed a low diversity. Genotype 13_A2 was the predominant in the population with a frequency of 67 % followed by 2_A1 (21 %) and 23_A1 (5 %). Genotype 23_A1 was detected only in tomato and potato isolates collected in 2013 and 2014.

Original language	English
Pages (from-to)	467-477
Number of pages	11
Journal	Fungal Biology
Volume	121
Issue number	5
Early online date	6 Feb 2017
DOIs	https://doi.org/10.1016/j.funbio.2017.01.004
Publication status	Published - May 2017

Keywords

Late blight
Mating type
Metalaxyl
Potato
SSR markers
Tomato

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@article{5d86854fdf80408b88032aec61888b4b,

title = "Characterization of Phytophthora infestans populations in northwestern Algeria during 2008-2014",

abstract = "A total of 161 Phytophthora infestans isolates, collected from infected potato and tomato plants during 2008-2014, were characterized based on mating type, metalaxyl sensitivity and polymorphism at 12 simple sequence repeat (SSR) loci, in order to investigate the population of P. infestans in the north-west of Algeria, an emerging potato production region. The majority of isolates were of A2 mating type (112 isolates). A high percentage (89 %) of resistance to metalaxyl among isolates was detected. The metalaxyl resistant phenotype was present in both mating types with a higher percentage in A2 mating type isolates. SSR-based genotypic analysis of P. infestans population showed a low diversity. Genotype 13_A2 was the predominant in the population with a frequency of 67 % followed by 2_A1 (21 %) and 23_A1 (5 %). Genotype 23_A1 was detected only in tomato and potato isolates collected in 2013 and 2014.",

keywords = "Late blight, Mating type, Metalaxyl, Potato, SSR markers, Tomato",

author = "Rekad, {Fatma Zohra} and Cooke, {David Edward Llewelyn} and Ivana Puglisi and Eva Randall and Yamina Guenaoui and Zouaoui Bouznad and Maria Evoli and Antonella Pane and Leonardo Schena and {Magnano di San Lio}, Gaetano and Cacciola, {Santa Olga}",

note = "This research was supported in principal by the University of Mostaganem, Algeria and a part of this work was funded by the Scottish Government.",

year = "2017",

month = may,

doi = "10.1016/j.funbio.2017.01.004",

language = "English",

volume = "121",

pages = "467--477",

journal = "Fungal Biology",

issn = "1878-6146",

publisher = "Elsevier",

number = "5",

}

TY - JOUR

T1 - Characterization of Phytophthora infestans populations in northwestern Algeria during 2008-2014

AU - Rekad, Fatma Zohra

AU - Cooke, David Edward Llewelyn

AU - Puglisi, Ivana

AU - Randall, Eva

AU - Guenaoui, Yamina

AU - Bouznad, Zouaoui

AU - Evoli, Maria

AU - Pane, Antonella

AU - Schena, Leonardo

AU - Magnano di San Lio, Gaetano

AU - Cacciola, Santa Olga

N1 - This research was supported in principal by the University of Mostaganem, Algeria and a part of this work was funded by the Scottish Government.

PY - 2017/5

Y1 - 2017/5

N2 - A total of 161 Phytophthora infestans isolates, collected from infected potato and tomato plants during 2008-2014, were characterized based on mating type, metalaxyl sensitivity and polymorphism at 12 simple sequence repeat (SSR) loci, in order to investigate the population of P. infestans in the north-west of Algeria, an emerging potato production region. The majority of isolates were of A2 mating type (112 isolates). A high percentage (89 %) of resistance to metalaxyl among isolates was detected. The metalaxyl resistant phenotype was present in both mating types with a higher percentage in A2 mating type isolates. SSR-based genotypic analysis of P. infestans population showed a low diversity. Genotype 13_A2 was the predominant in the population with a frequency of 67 % followed by 2_A1 (21 %) and 23_A1 (5 %). Genotype 23_A1 was detected only in tomato and potato isolates collected in 2013 and 2014.

AB - A total of 161 Phytophthora infestans isolates, collected from infected potato and tomato plants during 2008-2014, were characterized based on mating type, metalaxyl sensitivity and polymorphism at 12 simple sequence repeat (SSR) loci, in order to investigate the population of P. infestans in the north-west of Algeria, an emerging potato production region. The majority of isolates were of A2 mating type (112 isolates). A high percentage (89 %) of resistance to metalaxyl among isolates was detected. The metalaxyl resistant phenotype was present in both mating types with a higher percentage in A2 mating type isolates. SSR-based genotypic analysis of P. infestans population showed a low diversity. Genotype 13_A2 was the predominant in the population with a frequency of 67 % followed by 2_A1 (21 %) and 23_A1 (5 %). Genotype 23_A1 was detected only in tomato and potato isolates collected in 2013 and 2014.

KW - Late blight

KW - Mating type

KW - Metalaxyl

KW - Potato

KW - SSR markers

KW - Tomato

U2 - 10.1016/j.funbio.2017.01.004

DO - 10.1016/j.funbio.2017.01.004

M3 - Article

C2 - 28390504

SN - 1878-6146

VL - 121

SP - 467

EP - 477

JO - Fungal Biology

JF - Fungal Biology

IS - 5

ER -

Characterization of Phytophthora infestans populations in northwestern Algeria during 2008-2014

Abstract

Keywords

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