@inbook{4750df8ed15b4c1a9d823557c6145d72,
title = "Characterization of Protein Complexes Using Chemical Cross-Linking Coupled Electrospray Mass Spectrometry",
abstract = "Identification and characterization of large protein complexes is a mainstay of biochemical toolboxes. Utilization of cross-linking chemicals can facilitate the capture and identification of transient or weak interactions of a transient nature (Huang and Kim, PloS One 8:e61430, 2013; Gao et al., J Vis Exp doi: 10.3791/51387, 2014). Here we describe a detailed methodology for a cell culture-based proteomic approach. We describe the generation of cells stably expressing green fluorescent protein (GFP)-tagged proteins under the tetracycline-inducible promoter and subsequent proteomic analysis of GFP-interacting proteins. We include a list of proteins that were identified as interactors of GFP.",
keywords = "GFP affinity purification, Inducible expression, Interactome, Mass spectrometry, Protein complex, Protein–protein interaction",
author = "Cummins, {Timothy D.} and Sapkota, {Gopal P.}",
note = "{\textcopyright} Springer Science+Business Media New York 2017.",
year = "2018",
month = jan,
day = "1",
doi = "10.1007/7651_2017_85",
language = "English",
isbn = "9781493978526",
volume = "1788",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "43--61",
editor = "M. Sarwal and T. Sigel",
booktitle = "Tissue Proteomics",
address = "United States",
}