Characterization of resistance genes against scald (Rhynchosporium secalis (Oudem.) J.J. Davis) in barley (Hordeum vulgare L.) lines from central Norway, by means of genetic markers and pathotype tests

L. Reitan, S. Grønnerød, T. P. Ristad, S. Salamati, H. Skinnes, R. Waugh, Å Bjørnstad

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Rhynchosporium secalis is a serious pathogen of barley (Hordeum vulgare L.) in central Norway. A breeding effort was initiated in 1977 to introduce resistance from different sources into adapted genotypes, and the first cultivar from the program was recently released. However, little is known about the resistance genes introgressed in this cultivar or in advanced breeding lines. An effort was made to address this issue through a set of isolates and available molecular markers. Fourteen breeding lines and their resistance donors were investigated by evaluating their reactions to 11 R. secalis isolates. Bulked segregant analysis was used to identify molecular markers linked to resistance genes in 12 of the breeding lines. The isolates were found to be of less discriminating value than the markers. Useful information has been obtained as to the nature of several of the resistance genes introgressed. Eight of the 12 breeding lines contained introgressed genes that were located at the 'complex Rh' locus on chromosome 3H and hence may not easily be pyramided into the same genotype. Previous information about the nature of the resistance in 'Jet' is questioned. Neither of the resistance genes Rh or Rh2 seems to have been incorporated into Norwegian breeding material.

Original languageEnglish
Pages (from-to)31-39
Number of pages9
JournalEuphytica
Volume123
Issue number1
DOIs
Publication statusPublished - 4 Apr 2002

Fingerprint

Rhynchosporium secalis
pathotypes
Hordeum
Norway
Genetic Markers
Hordeum vulgare
Breeding
breeding lines
barley
genetic markers
Genes
genes
testing
Genotype
genotype
breeding
cultivars
chromosomes
Chromosomes
loci

Keywords

  • Breeding
  • Bulk segregant analysis
  • Genetic markers
  • Hordeum vulgare
  • Inoculation tests
  • Rhynchosporium secalis

Cite this

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abstract = "Rhynchosporium secalis is a serious pathogen of barley (Hordeum vulgare L.) in central Norway. A breeding effort was initiated in 1977 to introduce resistance from different sources into adapted genotypes, and the first cultivar from the program was recently released. However, little is known about the resistance genes introgressed in this cultivar or in advanced breeding lines. An effort was made to address this issue through a set of isolates and available molecular markers. Fourteen breeding lines and their resistance donors were investigated by evaluating their reactions to 11 R. secalis isolates. Bulked segregant analysis was used to identify molecular markers linked to resistance genes in 12 of the breeding lines. The isolates were found to be of less discriminating value than the markers. Useful information has been obtained as to the nature of several of the resistance genes introgressed. Eight of the 12 breeding lines contained introgressed genes that were located at the 'complex Rh' locus on chromosome 3H and hence may not easily be pyramided into the same genotype. Previous information about the nature of the resistance in 'Jet' is questioned. Neither of the resistance genes Rh or Rh2 seems to have been incorporated into Norwegian breeding material.",
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Characterization of resistance genes against scald (Rhynchosporium secalis (Oudem.) J.J. Davis) in barley (Hordeum vulgare L.) lines from central Norway, by means of genetic markers and pathotype tests. / Reitan, L.; Grønnerød, S.; Ristad, T. P.; Salamati, S.; Skinnes, H.; Waugh, R.; Bjørnstad, Å.

In: Euphytica, Vol. 123, No. 1, 04.04.2002, p. 31-39.

Research output: Contribution to journalArticle

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