TY - JOUR
T1 - Characterization of the cross-reacting determinant (crd) of the glycosyl-phosphatidylinositol membrane anchor of trypanosoma-brucei variant surface glycoprotein
AU - Zamze, Susanne E.
AU - Ferguson, Michael A. J.
AU - Collins, Ruth
AU - Dwek, Raymond A.
AU - Rademacher, Thomas W.
PY - 1988/10
Y1 - 1988/10
N2 - The cross-reacting determinant (CRD epitope) of the glycosyl-phosphatidylinositol (GPI) membrane anchor of Tryponosoma brucei glycoprotein has been analysed by selective chemical and enzymic modification of the isolated GPI structure combined with the use of a competitive ELISA inhibition assay for the detection of CRD epitopes. The data show that the CRD consists of at least three overlapping epitopes involving different regions of the molecule including the inositol 1, 2-cyclic phosphate, the non-N-acetylated-glucosamine residue and the galactose branch. Although the presence of all three of these structural features is required for quantitative binding of anti-CRD antibodies in ELISA and Western blotting, the Western blot reaction obtained inthe presence of any one epitope is still significant. The use of anti-CRD antibodies for the detection of GPI anchors is discussed.
AB - The cross-reacting determinant (CRD epitope) of the glycosyl-phosphatidylinositol (GPI) membrane anchor of Tryponosoma brucei glycoprotein has been analysed by selective chemical and enzymic modification of the isolated GPI structure combined with the use of a competitive ELISA inhibition assay for the detection of CRD epitopes. The data show that the CRD consists of at least three overlapping epitopes involving different regions of the molecule including the inositol 1, 2-cyclic phosphate, the non-N-acetylated-glucosamine residue and the galactose branch. Although the presence of all three of these structural features is required for quantitative binding of anti-CRD antibodies in ELISA and Western blotting, the Western blot reaction obtained inthe presence of any one epitope is still significant. The use of anti-CRD antibodies for the detection of GPI anchors is discussed.
U2 - 10.1111/j.1432-1033.1988.tb14310.x
DO - 10.1111/j.1432-1033.1988.tb14310.x
M3 - Article
SN - 0014-2956
VL - 176
SP - 527
EP - 534
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
IS - 3
ER -