Characterization of the human patatin-like phospholipase family

Paul A. Wilson (Lead / Corresponding author), Scott D. Gardner, Natalie M. Lambie, Stephane A. Commans, Daniel J. Crowther

Research output: Contribution to journalArticle

180 Citations (Scopus)

Abstract

Several publications have described biological roles for human patatin-like phospholipases (PNPLAs) in the regulation of adipocyte differentiation. Here, we report on the characterization and expression profiling of 10 human PNPLAs. A variety of bioinformatics approaches were used to identify and characterize all PNPLAs encoded by the human genome. The genes described represent a divergent family, most with a highly conserved ortholog in several mammalian species. In silico characterization predicts that two of the genes function as integral membrane proteins and are regulated by cAMP/cGMP. A structurally guided protein alignment of the patatin-like domain identifies a number of conserved residues in all family members. Quantitative PCR was used to determine the expression profile of each family member. Affymetrix-based profiling of a human preadipocyte cell line identified several members that are differentially regulated during cell differentiation. Cumulative data suggest that patatin-like genes normally expressed at very low levels are induced in response to environmental signals. Given the observed conservation of the patatin fold and lipase motif in all human PNPLAs, a single nomenclature to describe the PNPLA family is proposed.

Original languageEnglish
Pages (from-to)1940-1949
Number of pages10
JournalJournal of Lipid Research
Volume47
Issue number9
Early online date25 Jun 2006
DOIs
Publication statusPublished - 22 Sep 2006

Keywords

  • Adipocyte
  • Adiponutrin
  • Affymetrix
  • Desnutrin
  • Intracellular membrane-associated calcium-independent phospholipase A2γ
  • Neuropathy target esterase
  • Patatin-like phospholipase
  • Phospholipase A2, group VI
  • TaqMan

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