Characterization of the Xenopus replication licensing system

James P. J. Chong, Pia Thömmes, Alison Rowles, Hiro M. Mahbubani, J. Julian Blow

    Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

    71 Citations (Scopus)


    This chapter discusses the characterization of the xenopus replication licensing system. In eukaryotes the entire genome is replicated precisely once during each S phase of the cell cycle. Cell-free extracts of Xenopus eggs support chromosomal DNA replication in vitro, apparently under the same cell cycle controls that exist in vivo. When DNA is added to the cell-free system it is assembled into chromatin, and then into structures resembling normal interphase nuclei, processes that is necessary before the initiation of DNA replication can occur. The precise duplication of chromosomal DNA in the Xenopus cell-free system requires many thousands of replication origins to each fire once and only once. To achieve this, two distinct signals are required for initiation to occur at any given replication origin.
    Original languageEnglish
    Title of host publicationCell cycle control
    EditorsWilliam G. Dunphy
    PublisherAcademic Press
    Number of pages16
    ISBN (Print)9780121821845
    Publication statusPublished - 1997

    Publication series

    NameMethods in Enzymology
    PublisherAcademic Press


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