Characterization of the ligand binding site of the bovine IgA Fc receptor (bFcαR)

TY - JOUR

T1 - Characterization of the ligand binding site of the bovine IgA Fc receptor (bFcαR)

AU - Morton, H. Craig

AU - Pleass, Richard J.

AU - Woof, Jenny M.

AU - Brandtzaeg, Per

PY - 2004/12/24

Y1 - 2004/12/24

N2 - Recently, we identified a bovine IgA Fc receptor (bFcαR), which shows high homology to the human myeloid FcαR, CD89. IgA binding has previously been shown to depend on several specific residues located in the B-C and F-G loops of the membrane-distal extracellular domain 1 of CD89. To compare the ligand binding properties of these two FcαRs, we have mapped the IgA binding site of bFcαR. We show that, in common with CD89, Tyr-35 in the B-C loop is essential for IgA binding. However, in contrast to earlier observations on CD89, mutation of residues in the F-G loop did not significantly inhibit IgA binding.

AB - Recently, we identified a bovine IgA Fc receptor (bFcαR), which shows high homology to the human myeloid FcαR, CD89. IgA binding has previously been shown to depend on several specific residues located in the B-C and F-G loops of the membrane-distal extracellular domain 1 of CD89. To compare the ligand binding properties of these two FcαRs, we have mapped the IgA binding site of bFcαR. We show that, in common with CD89, Tyr-35 in the B-C loop is essential for IgA binding. However, in contrast to earlier observations on CD89, mutation of residues in the F-G loop did not significantly inhibit IgA binding.

UR - https://www.scopus.com/pages/publications/11144237492

U2 - 10.1074/jbc.M407807200

DO - 10.1074/jbc.M407807200

M3 - Article

C2 - 15485844

AN - SCOPUS:11144237492

SN - 0021-9258

VL - 279

SP - 54018

EP - 54022

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 52

ER -