Projects per year
Abstract
Trypanosoma theileri maintains a long-term extracellular infection with a low parasitaemia in bovids. The surface of this parasite is predicted to be decorated with several surface molecules including membrane surface proteases (MSPs), trans-sialidases and T. theileri putative surface proteins (TTPSPs). However, there are no experimental data to verify this hypothesis. Here, we have purified and partially characterized the surface glycoconjugates of T. theileri using biochemical and mass spectrometry-based approaches. The glycoconjugates fall into two classes: glycoproteins and glycolipids. Proteomic analysis of the glycoprotein fraction demonstrated the presence of MSPs and abundant mucin-like TTPSPs, with most predicted to be GPI-anchored. Mass spectrometric characterization of the glycolipid fraction showed that these are mannose- and galactose-containing glycoinositolphospholipids (GIPLs) that are larger and more diverse than those of its phylogenetic relative T. cruzi, containing up to 10 hexose residues and carrying either alkylacyl-phosphatidylinositol or inositol-phospho-ceramide (IPC) lipid components.
Original language | English |
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Article number | 111591 |
Number of pages | 9 |
Journal | Molecular and Biochemical Parasitology |
Volume | 256 |
Early online date | 29 Aug 2023 |
DOIs | |
Publication status | Published - Dec 2023 |
Keywords
- Trypanosoma theileri
- Glycoconjugate
- Glycoinositolphospholipids
- Glycosylphosphatidylinositol
- Mucin
- Mass-spectrometry
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Dive into the research topics of 'Characterization of the major surface glycoconjugates of Trypanosoma theileri'. Together they form a unique fingerprint.Projects
- 1 Finished
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Protein Glycosylation in Trypanosomes: Defining and Exploiting a Biological System (Senior Investigator Award)
Ferguson, M. (Investigator)
1/10/13 → 30/06/23
Project: Research
Equipment
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Fingerprints Proteomics Facility
Centre for Advanced Scientific TechnologiesFacility/equipment: Facility