Chemical Transformation of Yeast

Megan Bergkessel, Christine Guthrie

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Citations (Scopus)


Transformation of chemically competent yeast cells is a method for introducing exogenous DNA into living cells. Typically, the DNA is either a plasmid carrying an autonomous replication sequence that allows for propagation or a linear piece of DNA to be integrated into the genome. The DNA usually also carries a marker that allows for selection of successfully transformed cells by plating on the appropriate selective media.

Original languageEnglish
Title of host publicationLaboratory Methods in Enzymology
Subtitle of host publicationDNA
EditorsJon Lorsch
PublisherAcademic Press Inc.
Number of pages10
ISBN (Print)9780124186873
Publication statusPublished - 2013

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988


  • Autonomous replication sequence
  • Competent cells preparation
  • Polymerase chain reaction (PCR)
  • Transformation of yeast cells
  • YPAD medium

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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