@inbook{1f2963d3a7524fd98d4259fc66ba2a5d,
title = "Chemical Transformation of Yeast",
abstract = "Transformation of chemically competent yeast cells is a method for introducing exogenous DNA into living cells. Typically, the DNA is either a plasmid carrying an autonomous replication sequence that allows for propagation or a linear piece of DNA to be integrated into the genome. The DNA usually also carries a marker that allows for selection of successfully transformed cells by plating on the appropriate selective media.",
keywords = "Autonomous replication sequence, Competent cells preparation, Polymerase chain reaction (PCR), Transformation of yeast cells, YPAD medium",
author = "Megan Bergkessel and Christine Guthrie",
year = "2013",
doi = "10.1016/B978-0-12-418687-3.00026-4",
language = "English",
isbn = "9780124186873",
volume = "529",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "311--320",
editor = "Jon Lorsch",
booktitle = "Laboratory Methods in Enzymology",
address = "United States",
}