Projects per year
Abstract
Aquaglyceroporin-2 is a known determinant of melarsoprol-pentamidine cross-resistance in Trypanosoma brucei brucei laboratory strains. Recently, chimerization at the AQP2-AQP3 tandem locus was described from melarsoprol-pentamidine cross-resistant Trypanosoma brucei gambiense isolates from sleeping sickness patients in the Democratic Republic of the Congo. Here, we demonstrate that reintroduction of wild-type AQP2 into one of these isolates fully restores drug susceptibility while expression of the chimeric AQP2/3 gene in aqp2-aqp3 null T. b. brucei does not. This proves that AQP2-AQP3 chimerization is the cause of melarsoprol-pentamidine cross-resistance in the T. b. gambiense isolates.
Original language | English |
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Pages (from-to) | 65-68 |
Number of pages | 4 |
Journal | International Journal for Parasitology: Drugs and Drug Resistance |
Volume | 5 |
Issue number | 2 |
DOIs | |
Publication status | Published - Aug 2015 |
Keywords
- Aquaporin
- Drug resistance
- Human African trypanosomiasis
- Melarsoprol
- Pentamidine
- Reverse genetics
- Sleeping sickness
- Trypanosoma brucei gambiense
ASJC Scopus subject areas
- Infectious Diseases
- Pharmacology (medical)
- Pharmacology
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Dive into the research topics of 'Chimerization at the AQP2-AQP3 locus is the genetic basis of melarsoprol-pentamidine cross-resistance in clinical Trypanosoma brucei gambiense isolates'. Together they form a unique fingerprint.Projects
- 2 Finished
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Melarsoprol-Pentamidine Cross Resistance in African Trypanosomes.
Horn, D. (Investigator)
28/10/13 → 27/10/16
Project: Research
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High-Throughput Decoding of Virulence Mechanisms in African Trypanosomes (Senior Investigator Award)
Horn, D. (Investigator)
1/09/13 → 29/02/20
Project: Research