ChIP-Sequencing of TET Proteins

Kasper D. Rasmussen, Kristian Helin (Lead / Corresponding author)

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

Abstract

TET proteins are methylcytosine dioxygenases that interact directly with chromatin to shape the DNA methylation landscape. To increase the understanding of TET protein function in a specific cellular context, it is important to be able to map the interactions between TET proteins and DNA. This ChIP-seq protocol details our procedure to analyze TET2 bound DNA in disuccinimidyl glutarate (DSG) and formaldehyde-crosslinked chromatin but can also be adapted to study other TET enzymes.

Original languageEnglish
Title of host publicationTET Proteins and DNA Demethylation
Subtitle of host publicationMethods and Protocols
EditorsOzren Bogdanovic, Michiel Vermeulen
Place of PublicationNew York
PublisherHumana Press
Pages251-262
Number of pages12
Edition1
ISBN (Electronic)9781071612941
ISBN (Print)9781071612934 (hbk), 9781071612965 (pbk)
DOIs
Publication statusPublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2272
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • TET1
  • TET2
  • TET3
  • DNA methylation
  • DSG
  • ChIP-seq

Fingerprint

Dive into the research topics of 'ChIP-Sequencing of TET Proteins'. Together they form a unique fingerprint.

Cite this