Cholic acid binding by glutathione S-transferases from rat liver cytosol

TY - JOUR

T1 - Cholic acid binding by glutathione S-transferases from rat liver cytosol

AU - Hayes, J D

AU - Strange, R C

AU - Percy-Robb, I W

PY - 1980/1/1

Y1 - 1980/1/1

N2 - Cholic acid-binding activity in cytosol from rat livers appears to be mainly associated with enzymes having glutathione S-transferase activity; at least four of the enzymes in this group can bind the bile acid. Examination of the subunit compositions of different glutathione S-transferases indicated that cholic acid binding and the ability to conjugate reduced glutathione with 1,2-dichloro-4-nitrobenzene may be ascribed to different subunits.

AB - Cholic acid-binding activity in cytosol from rat livers appears to be mainly associated with enzymes having glutathione S-transferase activity; at least four of the enzymes in this group can bind the bile acid. Examination of the subunit compositions of different glutathione S-transferases indicated that cholic acid binding and the ability to conjugate reduced glutathione with 1,2-dichloro-4-nitrobenzene may be ascribed to different subunits.

KW - Animals

KW - Cholic Acids/metabolism

KW - Chromatography, Ion Exchange

KW - Cytosol/enzymology

KW - Dinitrochlorobenzene/metabolism

KW - Electrophoresis, Polyacrylamide Gel

KW - Glutathione Transferase/isolation & purification

KW - In Vitro Techniques

KW - Isoenzymes/isolation & purification

KW - Liver/enzymology

KW - Rats

UR - https://www.ncbi.nlm.nih.gov/pubmed/7378056

M3 - Article

C2 - 7378056

SN - 0264-6021

VL - 185

SP - 83

EP - 87

JO - Biochemical Journal

JF - Biochemical Journal

IS - 1

ER -