Chromatin accessibility landscapes activated by cell-surface and intracellular immune receptors

Pingtao Ding (Lead / Corresponding author), Toshiyuki Sakai, Ram Krishna Shrestha, Nicolas Manosalva Perez, Wenbin Guo, Bruno Pok Man Ngou, Shengbo He, Chang Liu, Xiaoqi Feng, Runxuan Zhang, Klaas Vandepoele, Dan MacLean, Jonathan D. G. Jones (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

Abstract

Activation of cell-surface and intracellular receptor-mediated immunity results in rapid transcriptional reprogramming that underpins disease resistance. However, the mechanisms by which co-activation of both immune systems lead to transcriptional changes are not clear. Here, we combine RNA-seq and ATAC-seq to define changes in gene expression and chromatin accessibility. Activation of cell-surface or intracellular receptor-mediated immunity, or both, increases chromatin accessibility at induced defense genes. Analysis of ATAC-seq and RNA-seq data combined with publicly available information on transcription factor DNA-binding motifs enabled comparison of individual gene regulatory networks activated by cell-surface or intracellular receptor-mediated immunity, and by both. These results and analyses reveal overlapping and conserved transcriptional regulatory mechanisms between the two immune systems.

Original languageEnglish
JournalJournal of Experimental Botany
Early online date13 Aug 2021
DOIs
Publication statusE-pub ahead of print - 13 Aug 2021

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